基于明胶—多孔炭过氧化氢生物传感器的制备及其应用研究

发布时间：2018-06-02 21:26

本文选题：明胶 + 多孔炭　；参考：《北京化工大学》2015年硕士论文

【摘要】：生物传感器的连续使用变得越来越重要,因为其构成元件稳定性的缺乏而导致长寿命的生物传感器未实现商业化。针对于此,本论文以明胶及鱼鳞基多孔炭材料为载体材料构建稳定性优异的生物传感器,并对其性能及应用方面做了系统研究。具体研究工作如下：第一,通过物理吸附和化学交联联合固定的方法对过氧化氢酶固定化技术的设计,成功制备出了高稳定性明胶-多孔炭固定化过氧化氢酶第三代生物传感器。明胶作为电极材料的分散剂与多孔炭形成稳定基体,良好的生物相容性确保了酶的生物活性。鱼鳞基多孔炭的多级孔分布结构和修饰电极敏感膜的多孔结构为离子的快速传递提供了通道,促进了酶与电极表面的直接电子传递。该酶传感器对H202表现出了良好的催化性能,其线性范围为0.2-10.7mM H2O2,检出限为0.7μM,灵敏度为74.69μAmM-1cm-2,抗干扰性好,具有优异的工作稳定性和长期储存稳定性。并将其应用于牛奶中H2O2的检测,其回收率在97%-103%。第二,以明胶为多功能助剂,抗坏血酸为还原剂还原硝酸银制备出了银纳米颗粒,并将其用于无酶传感器的构建,成功制备了高稳定性明胶／银纳米颗粒-多孔炭修饰的无酶过氧化氢传感器体系。鱼鳞基多孔炭不仅促进了银纳米颗粒与电极表面的直接电子传递,更是与明胶协同提高了其传感器的稳定性。该无酶传感器对H202检测的线性范围为0.2-12.3mM,检出限为0.6μM,灵敏度为228.92μAmM-1cm-2,且显示出了它优异的抗干扰性和长期储存稳定性。将其应用于牛奶中H202痕量检测,回收率控制在97%-103%,为H202的无酶测定提供了一个良好的平台。
[Abstract]:The continuous use of biosensors is becoming more and more important, because the stability of the components is lacking and the biosensors that lead to long life are not commercialized. In this paper, we use gelatin and fish scale based porous carbon materials as carrier materials to construct a biosensor with excellent stability, and make a series of performance and application aspects. The specific research work is as follows: first, a highly stable gelatin porous carbon immobilized catalase third generation biosensor has been successfully prepared by the method of physical adsorption and chemical crosslinking combined immobilization. Gelatin is used as the dispersant of the electrode material to form the stable basis of the porous carbon. The multilevel pore distribution structure of the porous carbon in the fish scale and the porous structure of the modified electrode sensitive membrane provide the channel for the rapid transfer of ions and promote the direct electron transfer between the enzyme and the surface of the electrode. The enzyme sensor has a good catalytic performance on the H202 table, and its linear range is in the linear range. 0.2-10.7mM H2O2, with a detection limit of 0.7 mu M, a sensitivity of 74.69 mu AmM-1cm-2, good interference resistance, excellent working stability and long-term storage stability, and its application in the detection of H2O2 in milk, its recovery rate is 97%-103%. second, gelatin is a multifunctional additive, and anti ascorbic acid is reduced as a reductant to prepare silver nanoparticles The particles are used for the construction of a non enzyme sensor, and a highly stable gelatin / silver nanoparticle - porous carbon free hydrogen peroxide sensor has been successfully prepared. The fish scale based porous carbon not only promotes the direct electron transfer of the silver nanoparticles and the electrode surface, but also improves the stability of the sensor in synergy with the gelatin. The linear range of H202 detection by enzyme sensor is 0.2-12.3mM, the detection limit is 0.6 mu M and the sensitivity is 228.92 AmM-1cm-2, and it shows its excellent anti-interference and long-term storage stability. It is applied to the trace detection of H202 in milk and the recovery rate is controlled in 97%-103%, which provides a good platform for the non enzyme determination of H202.
【学位授予单位】：北京化工大学
【学位级别】：硕士
【学位授予年份】：2015
【分类号】：TQ127.11;TP212.3

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