红枣根际复合功能菌的研制及肥效研究

发布时间：2018-02-01 08:56

本文关键词： 枣根际功能菌土壤养分果实品质　出处：《新疆农业大学》2015年硕士论文　论文类型：学位论文

【摘要】：红枣营养价值高,枣树栽培效益广,在新疆广为栽种,但是由于枣农缺乏相应的科学施肥指导,导致化肥用量多而没有实现增产,严重影响土壤肥力和生态安全。因此,大力发展微生物肥料,提高土壤肥力,维持生态平衡是当下急需解决的问题。本文选用新疆农业大学林业研究所微生物实验室提供的优良溶磷菌和解钾菌,分别对其活力进行测定、优化组合实验,初步确定出效果良好的菌株组合,并且对菌群在田间的肥效进行研究。获得如下结果:1.20株根际功能菌经过筛选分为P13、P15;P4、P8;K3、K7三组。并且各菌株可以分别混合而不发生拮抗作用。复合菌群在120 d后活菌数下降,并且发生污染现象。2.复合菌群对红枣根际土壤养分有显著影响。在不同生长时期,各处理养分在坐果期增加明显。P13P8K3的土壤碱解氮含量最高,比CK增加24.74 mg/kg;P15P8K3的土壤速效磷含量最高,比CK增加14.12 mg/kg;P13P4K3、P15P4K3的土壤速效钾含量迅速增加,比CK增加42.67%、39.43%;各处理的土壤有机质含量与CK差别不明显;果实成熟期,P13P4K3、P15P8K7的土壤pH明显下降到7.54,CK为7.82。3.复合菌群对红枣根际土壤微生物数量的影响显示:P13P4K3、P13P8K3的细菌数量显著高于CK;真菌数量比CK减少1.40%~28.77%;微生物总量变化与细菌变化规律相一致。根据主成分分析,复合菌群的综合得分依次为P13P8K3P15P8K3P15P4K3P13P4K3P13P4K7P15P4K7P13P8K7P15P8K7CK。4.复合菌群对红枣果实品质有影响。P13P4K7的总糖含量最高;P15P4K3和P15P8K7的总酸增幅最大,达到28.19%和33.14%;P13P4K7的Vc含量最高,为314.61 mg/100g;P15P4K7的蛋白质含量最高,比CK高4.16%;P15P4K3的环磷酸腺苷含量最高,为57.94μg/g,并且环磷酸腺苷/环磷酸鸟苷的比值最高,为3.81;P15P4K7的环磷酸鸟苷含量最高,为15.87μg/g。
[Abstract]:Jujube has high nutritional value and wide benefit of jujube tree cultivation. It is widely planted in Xinjiang. However, due to the lack of scientific fertilization guidance for jujube farmers, the amount of chemical fertilizer has not been increased. Soil fertility and ecological security are seriously affected. Therefore, microbial fertilizer should be developed to improve soil fertility. It is urgent to solve the problem of maintaining ecological balance at present. In this paper, we select the excellent phosphorus soluble bacteria and potassium lytic bacteria provided by the microbiology laboratory of Xinjiang Agricultural University Forestry Research Institute to determine their activities and optimize the combination experiment. The combination of strains with good effect was preliminarily determined, and the fertilizer efficiency of microflora in the field was studied. The following results were obtained: 1.20 strains of rhizosphere functional bacteria were screened and divided into P13 and P15; P4, P8; K3 + K7 group. And each strain could be mixed without antagonism. The number of living bacteria decreased after 120 days. The compound flora had significant effect on soil nutrients of red jujube rhizosphere. In different growth stages, the soil alkali-hydrolyzed nitrogen content of different treatments increased significantly at fruiting stage. P13P8K3 had the highest content of alkali-hydrolyzed nitrogen. Compared with CK, it increased 24.74 mg / kg; P15P8K3 had the highest available phosphorus content, which was 14.12 mg / kg higher than CK. The soil available potassium content of P13P4K3 and P15P4K3 increased rapidly, which was 42.67 and 39.43 than CK. There was no significant difference between the soil organic matter content of each treatment and CK. The soil pH of P13P4K3 P15P8K7 decreased to 7.54 during fruit ripening. CK was 7.82.3. The effect of complex microflora on the microorganism count in rhizosphere soil of jujube jujube showed that the number of bacteria in the rhizosphere soil of Zizyphus jujuba was significantly higher than that of P13P4K3P13P8K3. The number of fungi was 1.40% and 28.77% lower than that of CK. The change of the total amount of microorganism is consistent with that of bacteria. The comprehensive score of the complex flora was P13P8K3P15P8K3P13P4K3P13P4K7P15P4K7P13P8K7P15P8K7P15P8K7. The total sugar content of P13P4K7 was the highest. The total acids of P15P4K3 and P15P8K7 were the largest, reaching 28.19% and 33.14; The VC content of P13P4K7 was the highest, 314.61 mg / 100 g; The protein content of P15P4K7 was the highest, 4.16% higher than CK. The content of cyclic adenosine monophosphate (57.94 渭 g / g) was the highest in P15P4K3, and the ratio of cyclic adenosine monophosphate to cyclic guanosine monophosphate was the highest (3.81). The content of cyclic guanosine monophosphate in P15P4K7 was the highest (15.87 渭 g / g).
【学位授予单位】：新疆农业大学
【学位级别】：硕士
【学位授予年份】：2015
【分类号】：S665.1;S144

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