连作对烟草根际土壤细菌碳代谢指纹的影响

发布时间：2018-03-17 02:07

本文选题：烟草　切入点：连作　出处：《河北农业大学学报》2017年05期 　论文类型：期刊论文

【摘要】：为了探究连作对烟草根系微生态的影响,采用Biolog技术分析了不同连作年限下烟草根际土壤细菌的碳代谢指纹差异。结果表明:根际土壤细菌对31种碳源的AWCD值随连作年限的增加而下降,与CK相比,连作5,10年后分别下降12.7%,74.0%;但6类不同碳源之间AWCD值降幅差异较大,连作5,10年后,降幅分别为3.7%~52.1%,64.9%~80.3%;主成分分析表明,以糖类、氨基酸类碳源利用为主的细菌数量显著减少是导致碳源总体利用能力下降的主要因素;连作改变了烟草根际细菌的代谢特征,表现为对糖类、氨基酸类碳源利用率下降,对多聚物类碳源利用率增加;连作降低了根际土壤细菌的香浓指数(丰富度)。
[Abstract]:In order to explore the effect of continuous cropping on tobacco root microecology, Biolog technique was used to analyze the difference of carbon metabolism fingerprints of bacteria in tobacco rhizosphere soil under different cropping years. The results showed that the AWCD value of 31 carbon sources decreased with the increase of continuous cropping years, compared with CK. After 5 years of continuous cropping and 10 years of continuous cropping, the value of AWCD decreased by 12.74.0, but there was a great difference between the six kinds of carbon sources. After 5years of continuous cropping, the decrease was 3.72.1and 64.9and 80.3.The principal component analysis showed that the main component analysis showed that, in the case of sugar, The significant decrease in the number of bacteria mainly using amino acid carbon sources was the main factor leading to the decrease of the total utilization capacity of carbon sources, and the metabolic characteristics of tobacco rhizosphere bacteria were changed by continuous cropping, which showed that the utilization ratio of carbon sources of amino acids and carbohydrates decreased. The utilization rate of carbon source of polymer was increased and the aroma index (richness) of bacteria in rhizosphere was decreased by continuous cropping.
【作者单位】：江西省红壤研究所/国家红壤改良工程技术研究中心;湖南农业大学教育学院;
【基金】：公益性行业农业科研专项(201203030)
【分类号】：S154.3;S572

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