乙草胺降解菌系的分离鉴定及其生长降解特性研究

发布时间：2018-06-11 12:15

本文选题：乙草胺 + 降解菌系　；参考：《基因组学与应用生物学》2017年11期

【摘要】：乙草胺是一种主要用于防除禾本科杂草的酰胺类除草剂,苄嘧磺隆是一种用于防除阔叶杂草的磺酰脲类除草剂,乙·苄复配制剂由于扩大了除草谱而被广泛应用于稻田中,但大量使用也带来了环境隐患。本研究从长期施用乙·苄复配制剂的稻田土壤中筛选到1个可耐受600 mg/L苄嘧磺隆的乙草胺降解复合菌系,从中分离到4个细菌菌株66C-1、66C-2、66C-3、66C-4,利用形态、生理生化及分子生物学方法,分别鉴定为粘质沙雷式菌(Serratia marcescens)、Psedomona shunanensis、恶臭假单胞菌(Pseudomonas putida)和硝化还原假单胞菌(Pseudomonas nitroreducens)。这4个菌株等比例混合构成的降解菌系HXDW可以利用乙草胺为唯一碳源、但不能利用丁草胺、异丙甲草胺和吡氟酰草胺等其他酰胺类除草剂。降解菌系HXDW在乙草胺初始浓度为100 mg/L的无机盐培养基中,0.1%~2.0%Na Cl浓度范围内生长良好,最适培养温度为25℃。提取降解菌系不同部位粗酶液对50 mg/L乙草胺进行处理,胞外提取液的24 h乙草胺降解率为44.95%,表明降解菌系HXDW对乙草胺的降解作用主要存在于胞外。
[Abstract]:Acetochlor is an amidic herbicide used mainly to prevent weeds from grasses. It is a sulfonylurea herbicide used to prevent broadleaf weeds. Ethyl benzyl compound is widely used in rice fields because of enlarging the weed control spectrum, but it has also brought about the hidden danger of the environment. This study has been used in the long term application of ethyl benzyl. 1 compound strains of acetochlor, which can tolerate 600 mg/L of 600 mg/L, were screened from the rice field soil, and 4 bacterial strains were isolated from the soil, which were identified as Serratia marcescens (Serratia marcescens), Psedomona shunanensis, Pseudomonas maloda (Pseu), respectively. Domonas putida) and nitrification reduction Pseudomonas (Pseudomonas nitroreducens). The biodegradation bacteria system composed of these 4 strains, HXDW can use acetochlor as the only carbon source, but can not use butachlor, formalamines and pyrochloramines and other amide herbicides. The Degradation Strain HXDW in acetochlor is 100 mg/L in the initial concentration. In the inorganic salt medium, the growth of 0.1%~2.0%Na Cl was good and the optimum culture temperature was 25. 50 mg/L acetochlor was treated with the crude enzyme solution from different parts of the degrading bacteria. The degradation rate of 24 h acetochlor was 44.95%, indicating that the degradation of acetochlor was mainly outside the cell.
【作者单位】：贵州大学生命科学学院;
【基金】：贵州省科学技术基金项目“苄嘧磺隆、乙草胺及其复配制剂的微生物降解和胁迫响应研究(黔科合J字[2014]2065号)”资助
【分类号】：X172;X592

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