黄土丘陵区人工林土壤微生物PLFA标记多样性分析
发布时间：2019-05-24 21:30
【摘要】：为研究不同植被下土壤微生物群落结构多样性的差异,采用磷脂脂肪酸(PLFA)生物标记法,对山西吉县黄土丘陵区典型人工林刺槐、油松以及荒草地的土壤微生物群落结构多样性进行分析研究。结果表明:不同植被下土壤微生物PLFA标记含量存在显著差异,刺槐人工林的土壤微生物PLFA总量最大,细菌、真菌、放线菌的PLFA含量均大于油松人工林和荒草地;细菌PLFA含量在3种样地中的差异显著;2种人工林的真菌PLFA含量接近,而与荒草地差异显著,与荒草地相比,人工林的种植可明显提高土壤中真菌的比例;革兰氏阳性菌与革兰氏阴性菌呈现相同的变化趋势,油松人工林与荒草地2种菌群的PLFA含量接近,而与刺槐人工林差异显著。通过相关性分析发现,不同植被下土壤细菌、真菌、革兰氏阳性菌与革兰氏阴性菌各总PLFA与土壤养分因子之间密切相关;不同植被下土壤微生物群落多样性差异显著,刺槐人工林更为丰富,且各个菌群分布更为均匀,油松人工林土壤的微生物群落多样性及菌群分布均匀度与刺槐人工林土壤较为接近,荒草地土壤微生物群落多样性和均匀程度较2种人工林明显偏低。因此从微生物群落结构多样性的角度看,人工林的种植能够明显改善微生物群落结构,改善土壤质量;刺槐人工林是黄土丘陵区人工植被恢复的较好选择。
[Abstract]:In order to study the diversity of soil microbial community structure under different vegetation, phospholipid fatty acid (PLFA) biomarker method was used to study Robinia pseudoacacia (Robinia pseudoacacia) in loess hilly region of Jixian County, Shanxi Province. The diversity of soil microbial community structure in Pinus tabulaeformis and wasteland was analyzed and studied. The results showed that there were significant differences in PLFA marker content of soil microorganisms under different vegetation. The total amount of soil microbial PLFA in Robinia pseudoacacia plantation was the largest, and the PLFA content of bacteria, fungi and actinomycetes was higher than that of Pinus tabulaeformis plantation and wasteland. The content of bacterial PLFA in the three plots was significantly different, and the content of fungal PLFA in the two plantations was close to that in the wasteland, but significantly different from that in the wasteland. Compared with the wasteland, the planting of the plantation could significantly increase the proportion of fungi in the soil. Gram-positive bacteria and Gram-negative bacteria showed the same change trend. The PLFA content of Pinus tabulaeformis plantation and wasteland was close to that of Robinia pseudoacacia plantation, but significantly different from that of Robinia pseudoacacia plantation. Through correlation analysis, it was found that the total PLFA of soil bacteria, fungi, Gram-positive bacteria and Gram-negative bacteria under different vegetation were closely related to soil nutrient factors. There were significant differences in soil microbial community diversity under different vegetation, Robinia pseudoacacia plantation was more abundant, and the distribution of each flora was more uniform. The soil microbial community diversity and flora distribution evenness of Pinus tabulaeformis plantation were close to those of Robinia pseudoacacia plantation, and the diversity and uniformity of soil microbial community of wasteland grassland were significantly lower than those of the two plantations. Therefore, from the point of view of microbial community structure diversity, plantation can obviously improve microbial community structure and soil quality, and Robinia pseudoacacia plantation is a good choice for artificial vegetation restoration in loess hilly region.
【作者单位】： 北京林业大学水土保持与荒漠化防治教育部重点实验室 山西吉县国家森林生态系统野外观测研究站;
【基金】：“十二五”国家科技支撑计划项目(2015BAD07B02) 国家生态系统观测研究网络运行服务项目(2014—2015)
【分类号】：S714.3
本文编号：2485192
[Abstract]:In order to study the diversity of soil microbial community structure under different vegetation, phospholipid fatty acid (PLFA) biomarker method was used to study Robinia pseudoacacia (Robinia pseudoacacia) in loess hilly region of Jixian County, Shanxi Province. The diversity of soil microbial community structure in Pinus tabulaeformis and wasteland was analyzed and studied. The results showed that there were significant differences in PLFA marker content of soil microorganisms under different vegetation. The total amount of soil microbial PLFA in Robinia pseudoacacia plantation was the largest, and the PLFA content of bacteria, fungi and actinomycetes was higher than that of Pinus tabulaeformis plantation and wasteland. The content of bacterial PLFA in the three plots was significantly different, and the content of fungal PLFA in the two plantations was close to that in the wasteland, but significantly different from that in the wasteland. Compared with the wasteland, the planting of the plantation could significantly increase the proportion of fungi in the soil. Gram-positive bacteria and Gram-negative bacteria showed the same change trend. The PLFA content of Pinus tabulaeformis plantation and wasteland was close to that of Robinia pseudoacacia plantation, but significantly different from that of Robinia pseudoacacia plantation. Through correlation analysis, it was found that the total PLFA of soil bacteria, fungi, Gram-positive bacteria and Gram-negative bacteria under different vegetation were closely related to soil nutrient factors. There were significant differences in soil microbial community diversity under different vegetation, Robinia pseudoacacia plantation was more abundant, and the distribution of each flora was more uniform. The soil microbial community diversity and flora distribution evenness of Pinus tabulaeformis plantation were close to those of Robinia pseudoacacia plantation, and the diversity and uniformity of soil microbial community of wasteland grassland were significantly lower than those of the two plantations. Therefore, from the point of view of microbial community structure diversity, plantation can obviously improve microbial community structure and soil quality, and Robinia pseudoacacia plantation is a good choice for artificial vegetation restoration in loess hilly region.
【作者单位】： 北京林业大学水土保持与荒漠化防治教育部重点实验室 山西吉县国家森林生态系统野外观测研究站;
【基金】：“十二五”国家科技支撑计划项目(2015BAD07B02) 国家生态系统观测研究网络运行服务项目(2014—2015)
【分类号】：S714.3
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