锐孔法制备水牛乳活性肽微胶囊工艺优化及体外释放研究

发布时间：2018-04-20 19:02

本文选题：水牛乳活性肽 + 锐孔法　；参考：《食品工业科技》2017年08期

【摘要】：为了减少胃肠道对抗氧化活性肽的分解并使其具有肠道缓释效果,以海藻酸钠、壳聚糖为壁材,用锐孔法制备水牛乳活性肽微胶囊。通过响应面法优化了海藻酸钠溶液浓度、壳聚糖溶液浓度以及Ca~(2+)浓度的工艺条件,并对制备的微胶囊进行扫描电镜观察以及体外释放检测。结果表明:制备载水牛乳活性肽微胶囊的优化工艺为:海藻酸钠、壳聚糖和CaCl_2溶液的浓度分别为1.28%、1.51%和2.20%(w/v),在此工艺条件下,活性肽的包埋率可达95.20%。通过扫描电镜发现,海藻酸钠-壳聚糖复合壁材载肽结构完整紧实。体外缓释实验表明,样品在人工胃液(pH=2.0)中释放量为7.98%,在人工肠液(pH=6.8)中作用12 h后的相对累计释放量为89.41%,样品表现出耐酸和良好的模拟肠道环境缓释效果,制备的水牛乳活性肽微胶囊能够较好的保护目标肽、提高稳定性、耐酸和缓释效果。
[Abstract]:In order to reduce the decomposition of antioxidant peptides in gastrointestinal tract and make them have the effect of intestinal sustained release, the microcapsules of buffalo active peptides were prepared by sharp hole method with sodium alginate and chitosan as the wall materials. The technological conditions of sodium alginate solution concentration, chitosan solution concentration and Ca~(2 concentration were optimized by response surface method, and the microcapsules were observed by scanning electron microscope and in vitro release detection. The results showed that the optimum conditions for preparation of buffalo milk active peptide microcapsules were as follows: the concentration of sodium alginate, chitosan and CaCl_2 solution were 1.28% and 2.20%, respectively. Under these conditions, the entrapment rate of active peptide could reach 95.20%. It was found by scanning electron microscope that the structure of peptide loaded by sodium alginate-chitosan composite wall material was intact and compacted. The results of in vitro slow-release experiment showed that the release amount of the sample was 7.98 in the artificial gastric juice (pH ~ (2.0)) and 89.41 in the artificial intestinal fluid (pH ~ (6.8)). The sample showed acid resistance and good slow release effect in the simulated intestinal environment. The prepared buffalo milk active peptide microcapsule can protect the target peptide, improve the stability, acid resistance and slow release effect.
【作者单位】：广西大学轻工与食品工程学院;华南理工大学食品科学与工程学院;广西东盟食品药品安全检验检测中心;
【基金】：国家自然科学基金面上项目资助(31371762)
【分类号】：TQ936.16

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