三血管结扎法制备兔后循环缺血模型的评价

发布时间：2018-05-10 19:06

本文选题：后循环缺血 + 动物模型　；参考：《新疆医科大学》2010年硕士论文

【摘要】： 目的:评估三血管结扎法制备兔后循环缺血模型的可靠性。方法:新西兰大白兔108只,等分为术后15min、30min、120min各36只。上述时间的兔按性别、兔龄、体重配伍,再随机分到假手术组(12只),右侧颈总动脉结扎组(12只)和右侧颈总动脉加双侧椎动脉结扎(三血管结扎)组(12只)。各组于相应的时间进行生理学指标(心率、呼吸、瞳孔直径)的检查以及磁共振PWI、DWI检查。检查完后,每组取两只动物迅速断头取脑,按MRI扫描层面切片,将脑片置于2%的TTC溶液中染色。再取四只动物,心脏灌注4%的多聚甲醛后,其中两只取脑ROI做病理标本(HE染色),两只制备电镜标本(置于2.5%戊二醛中送检)。结果:各组于相应的时间生理学指标差异无统计学意义(P0.05);三血管结扎组于术后15min与假手术组、右侧颈总动脉结扎组比较,CBF下降,MTT延长,差异均有统计学意义(P0.001),CBV、ADC值差异均无统计学意义(P0.05);术后30min,CBF、CBV下降,MTT延长,差异均有统计学意义(P0.01),ADC值差异均无统计学意义(P0.05);术后120min,CBF、CBV下降,MTT延长,ADC值下降,差异均有统计学意义(P0.01)。右侧颈总动脉结扎组于术后不同时间与假手术组比较,CBF、CBV、MTT、ADC值差异均无统计学意义(P0.05)。不同时间各组DWI均无异常高信号。三血管结扎组电镜显示ROI细胞器异常,但未见神经元坏死改变,各组TTC、HE染色均未见异常改变。结论:三血管结扎法可以制备兔后循环缺血模型,但不能获得后循环梗死模型。
[Abstract]:Objective: to evaluate the reliability of rabbit model of posterior circulation ischemia by three-vessel ligation. Methods: 108 New Zealand white rabbits were divided into 36 rabbits at 15 min and 30 min after operation for 120 min. According to sex, age and body weight, rabbits were randomly divided into sham operation group (n = 12), right common carotid artery ligation group (n = 12) and right common carotid artery plus bilateral vertebral artery ligation (n = 12). The physiological indexes (heart rate, respiration, pupil diameter) and Mr PWI DWI were examined at the corresponding time. After the examination, two animals in each group were cut off quickly and their brains were sliced according to MRI scanning plane. The brain slices were stained in 2% TTC solution. After 4% paraformaldehyde was infused into the heart of four animals, two of them were taken from brain ROI for HE staining and two were prepared for electron microscope (placed in 2.5% glutaraldehyde for examination). Results: there was no significant difference in the physiological indexes between the three groups in the corresponding time, while in the tri-vessel ligation group, the 15min was significantly decreased compared with the sham operation group, and the right common carotid artery ligation group was longer than that in the right common carotid artery ligation group. There was no significant difference in ADC value between CBV and CBV, but no significant difference was found in the ADC value of CBFCBV, but the decrease of CBV in CBFCBV was prolonged at 30 min after operation, and the difference was not statistically significant (P 0.05), but the decrease of CBF CBV in 120 min after operation prolonged the decrease of ADC value (P 0.01), and the difference was statistically significant (P 0.01). There was no significant difference in ADC between right common carotid artery ligation group and sham operation group at different time after operation (P 0.05). No abnormal hyperintensity of DWI was observed in all groups at different time points. In the tri-vessel ligation group, the organelles of ROI were abnormal under electron microscope, but no changes of neuronal necrosis were found, and no abnormal changes were found in all groups. Conclusion: the rabbit model of posterior circulation ischemia can be made by three-vessel ligation, but the model of posterior circulation infarction can not be obtained.
【学位授予单位】：新疆医科大学
【学位级别】：硕士
【学位授予年份】：2010
【分类号】：R743.3

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