珠三角河网地区粪便污染源解析

发布时间：2018-03-29 10:39

本文选题：实时荧光定量PCR　切入点：微生物污染　出处：《中国环境科学》2017年09期

【摘要】：准确识别水体中微生物污染物的宿主来源是进行针对性污染治理的基础.应用实时荧光定量PCR(q PCR)技术筛选出适用于珠江三角洲河网地区的宿主特异性拟杆菌引物,并结合总细菌引物(BACT1369F/PROK1541R)、大肠埃希氏菌引物(EC23S857F/EC23S857R)、拟杆菌通用引物(Gen Bac3F/Gen Bac3R)及粪大肠菌群(Fecal coliform)和大肠埃希氏菌(Escherichia coli)等常规水质指标对研究区域的水体进行分析,结果表明:人源(q HS601F/q Bac725R)、牛源(Bac B2-590F/Bac708Rm)、猪源(Bac41F/Bac163R)及鸡源(q C160F-HU/q Bac265R-HU)特异性拟杆菌引物在珠江三角洲地区同时具有较高的灵敏度和较强的特异性,适用于目标研究区域.选取的14处城市地表水均受到粪便污染,污染源分别为人、反刍动物和禽类粪便.水体中各污染源强度为人源反刍动物鸡源,其中人源特异性拟杆菌浓度与粪大肠菌群(Faecal coliform)、大肠埃希氏菌(Escherichia coli)及大肠埃希氏菌引物(EC23S857F/EC23S857R)浓度具有较好的相关性(P0.05),说明人粪是各地表水中微生物污染的主要贡献源.
[Abstract]:Accurate identification of the host source of microbial pollutants in water is the basis of targeted pollution control. The host-specific bacteroid primers suitable for river network in the Pearl River Delta region were screened by real-time fluorescence quantitative PCR(q PCR. The water samples of the study area were analyzed by using the general water quality indexes, such as BACT1369F / PROK1541RN, Escherichia coli primer EC23S857Fr / EC23S857RN, Bacteroides universal primer Gen Bac3F/Gen Bac3R, fecal coliform and Escherichia coli. The results showed that the specific primers of human HS601F/q Bac725R, bovine B2-590F / Bac708RmN, porcine Bac41Fr / Bac163R) and chicken C160F-HU/q C160F-HU/q Bac265R-HUhad high sensitivity and strong specificity in the Pearl River Delta region at the same time. All of the 14 urban surface water were polluted by feces, the pollution sources were human, ruminant and poultry faeces, and the intensity of each pollution source in the water was human ruminant chicken source. There was a good correlation between the concentration of Human-specific Bacteroides and the concentrations of fecal coliform, Escherichia coli and EC23S857F / EC23S857R, indicating that human feces were the main contributors of microbial contamination in surface water.
【作者单位】：武汉理工大学资源与环境工程学院;环境保护部华南环境科学研究所;广东省水与大气污染防治重点实验室;武汉科技大学资源与环境工程学院;浙江工商大学环境科学与工程学院;
【基金】：国家自然科学基金项目(41303054) 中央级公益性科研院所基本科研业务专项
【分类号】：X52

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