Induction of human β-defensin-2 in airway epithelial cells b

发布时间：2021-12-30 03:35

　　Human β-definsin-2（hBD-2）is mainly induced by bacterial factors and pro-inflammation mediators in epithelial cells.As the major cause of community-acquired pneumonia,whether Streptococcus pneumoniae（S.pneumoniae）stimulation induces hBD-2 expression in airway epithelial cells is elusive.In this study,we found that S.pneumoniae stimulation induced hBD-2 expression in a time-and concentration-dependent manner in primary human airway epithelial cells.To further reveal the mechanism of S.pneumoniae i... 

【文章来源】：Chinese Science Bulletin. 2014,59(16)SCI

【文章页数】：7 页

【文章目录】：
1 Introduction
2 Materials and methods
    2.1 In vitro culturing of primary human airway epithelial cells
    2.2 Infection with S. pneumoniae
    2.3 RNA isolation, reverse transcription,and semiquantitative real-time PCR
    2.4 Electrophoretic mobility shift assay (EMSA)
    2.5 Intracellular free Ca2+measurements
    2.6 Statistical analysis
3 Results
expression in a time-and concentration-dependent manner in airway epithelial cells">    3.1 S. pneumoniae stimulation induced h BD-2 m RNAexpression in a time-and concentration-dependent manner in airway epithelial cells

    3.2 NF-j B activation is involved in the S. pneumoniaeinduced h BD-2 expression
in the S.pneumoniae-induced hBD-2 expression">    3.3 Intracellular Ca2+signaling participatedin the S.pneumoniae-induced hBD-2 expression

    3.4 Role of IP3 receptor in S. pneumoniae-induced h BD-2 expression
4 Discussion



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