咬合干扰致大鼠咬肌能量代谢产物含量变化
发布时间：2019-01-15 08:27
【摘要】：目的:观察咬合干扰后不同时间大鼠咬肌能量代谢产物腺嘌呤核苷三磷酸(adenosine triphosphate,ATP)、腺嘌呤核苷二磷酸(adenosine diphosphate,ADP)、次黄嘌呤核苷酸(inosine monophosphate,IMP)、磷酸肌酸、肌酸、乳酸及p H水平的变化,分析咬合干扰对咀嚼肌能量代谢的影响。方法:选用雄性Sprague-Dawley大鼠(220~250 g)50只,随机分为实验组(40只)和对照组(10只),实验组于右上第一磨牙粘固0.4 mm厚金属冠建立咬合干扰,并分别维持3、7、10、14 d(每个时间点各10只),对照组不施加咬合干扰。各组大鼠全麻下取双侧咬肌组织,其中5只大鼠样本加入0.4 mol/L高氯酸(10 m L/g)充分匀浆,离心、过滤后采用高效液相色谱分析ATP、ADP、IMP、磷酸肌酸、肌酸及乳酸含量,另外5只大鼠样本加入含5 mmol/L碘醋酸钠的匀浆液(10 m L/g),充分匀浆后在37℃恒温水浴环境中利用p H计测试p H值。结果:与对照组相比,大鼠双侧咬肌ATP含量在咬合干扰3 d[右侧:(5.36±0.13)μmol/g,左侧:(5.77±0.25)μmol/g]升高(P0.05),7、10和14 d没有显著改变;大鼠双侧咬肌IMP[右侧:(0.21±0.03)μmol/g,左侧:(0.19±0.03)μmol/g]、肌酸[右侧:(24.76±2.94)μmol/g,左侧:(27.75±2.23)μmol/g]含量在咬合干扰7 d升高(P0.05),3、10和14 d没有显著改变;大鼠双侧咬肌磷酸肌酸含量在咬合干扰7、10和14 d降低[右侧分别为:(10.70±0.71)μmol/g、(11.57±0.52)μmol/g、(10.74±1.39)μmol/g,左侧分别为:(10.05±0.57)μmol/g、(10.75±1.12)μmol/g、(10.61±1.15)μmol/g,P0.05],3 d没有显著改变;大鼠双侧咬肌ADP、乳酸含量及p H水平在咬合干扰后各时间点均没有显著改变(P0.05)。结论:咬合干扰导致大鼠咀嚼肌能量代谢产物含量改变,可能与咬合干扰诱发咀嚼肌疼痛、功能紊乱、肌纤维构筑改变等病理过程相关。
[Abstract]:Objective: to observe the energy metabolites of masseter muscle of rats at different time after occlusal interference: adenosine triphosphate (adenosine triphosphate,ATP), adenosine diphosphate (adenosine diphosphate,ADP), Hypoxanthine nucleotide (inosine monophosphate,IMP), creatine phosphate (creatine phosphate). The changes of lactic acid and pH levels and the effects of occlusal interference on energy metabolism of masticatory muscles were analyzed. Methods: 50 male Sprague-Dawley rats (220 250g) were randomly divided into two groups: experimental group (40 rats) and control group (10 rats). The occlusion interference was established in the right first molar with 0.4 mm thick metal crown. Ten minutes and 14 days (10 rats in each time point), the control group did not exert occlusion interference. Bilateral masseter muscle tissues were taken from rats in each group under general anesthesia. The samples of 5 rats were added with 0.4 mol/L perchloric acid (10 mL / g) to fully homogenate, centrifuged, filtered, and then analyzed by high performance liquid chromatography (HPLC) for creatine phosphate. The contents of creatine and lactic acid in the other 5 rats were added to the homogenate solution containing 5 mmol/L sodium iodide acetate (10 mL / g), and then pH was measured by pH meter in 37 鈩，
本文编号：2409042
[Abstract]:Objective: to observe the energy metabolites of masseter muscle of rats at different time after occlusal interference: adenosine triphosphate (adenosine triphosphate,ATP), adenosine diphosphate (adenosine diphosphate,ADP), Hypoxanthine nucleotide (inosine monophosphate,IMP), creatine phosphate (creatine phosphate). The changes of lactic acid and pH levels and the effects of occlusal interference on energy metabolism of masticatory muscles were analyzed. Methods: 50 male Sprague-Dawley rats (220 250g) were randomly divided into two groups: experimental group (40 rats) and control group (10 rats). The occlusion interference was established in the right first molar with 0.4 mm thick metal crown. Ten minutes and 14 days (10 rats in each time point), the control group did not exert occlusion interference. Bilateral masseter muscle tissues were taken from rats in each group under general anesthesia. The samples of 5 rats were added with 0.4 mol/L perchloric acid (10 mL / g) to fully homogenate, centrifuged, filtered, and then analyzed by high performance liquid chromatography (HPLC) for creatine phosphate. The contents of creatine and lactic acid in the other 5 rats were added to the homogenate solution containing 5 mmol/L sodium iodide acetate (10 mL / g), and then pH was measured by pH meter in 37 鈩，
本文编号：2409042
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