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Key genes expressed in different stages of spinal cord ische

发布时间:2023-12-24 15:06
  The temporal expression of microRNA after spinal cord ischemia/reperfusion injury is not yet fully understood.In the present study,we established a model of spinal cord ischemia in Sprague-Dawley rats by clamping the abdominal aorta for 90 minutes,before allowing reperfusion for 24 or 48 hours.A sham-operated group underwent surgery but the aorta was not clamped.The damaged spinal cord was removed for hematoxylin-eosin staining and RNA extraction.Neuronal degeneration and tissue edema were the m...

【文章页数】:6 页

【文章目录】:
Graphical Abstract
Introduction
Materials and Methods
    Animals
    Construction of SCIRI rat model
    Neurological function assessment
    Histological staining
    Extraction and identifi cation of RNA
    Rat SCIRI mi RNA expression profi le screening
    Statistical analysis
Results
    Neurological function of rats with SCIRI
    Histological changes of injured spinal cord in rats
    Extraction and identifi cation of RNA
    Screening of mi RNA expression profi le in rats with SCIRI
    Preliminary analysis of diff erentially expressed mi RNAs in rat models of SCIRI
Discussion



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