脾肾阳虚型肠易激综合征大鼠模型的建立与评价

发布时间：2018-04-06 01:27

本文选题：脾肾阳虚证　切入点：腹泻型肠易激综合征　出处：《中国中西医结合杂志》2017年08期

【摘要】：目的探讨脾肾阳虚型肠易激综合征(IBS-D)病证结合大鼠模型的建立方法。方法 4周龄雄性SD大鼠38只,随机分为正常组10只、模型组14只和温肾健脾方组14只,采用物理化学刺激共同作用的方法,即全周期(4周)番泻叶灌胃联合2周冰醋酸灌肠+固定器束缚、2周球囊直肠刺激+夹尾刺激制备大鼠模型,造模后温肾健脾方组以13.98 g/(kg·d)温肾健脾方灌胃给药2周,模型组同期予等容量蒸馏水灌胃,正常组无干预。观察大鼠形态学表现和体重;比较排便粒数、粪便含水量、Bristol分型,评估肠道敏感性、肠道动力,并观察肠道黏膜病理组织学。结果与正常组比较,模型组精神萎靡,易激惹,体重无明显变化(P0.05),排便粒数明显增多(P0.05),粪便含水量增加(P0.05),Bristol分型中5、6、7型明显增多,肠道敏感性、肠道动力增加(P0.05);与模型组比较,温肾健脾方组动作较灵活,偶见烦躁,体重无明显变化(P0.05),排便粒数下降(P0.05),粪便含水量减少(P0.05),Bristol分型向4型转化,肠道敏感性、肠道动力下降(P0.05)。各组大鼠肠道黏膜病理组织学表现均未见明显异常。结论本物理化学刺激共同作用的造模方法可作为制备脾肾阳虚证IBS-D大鼠模型较理想的方法。
[Abstract]:Objective to explore the method of establishing the model of IBS-Dsyndrome combined with spleen and kidney yang deficiency syndrome in rats.Methods Thirty-eight 4-week-old male Sprague-Dawley rats were randomly divided into normal group (n = 10), model group (n = 14) and Wenshen Jianpi group (n = 14).The rat model was made by gastric perfusion of Senna leaf and 2 weeks of frozen acetic acid enema fixator for 2 weeks. After the model was made, Wenshen Jianpi prescription group was given intragastric administration of Wenshen Jianpi prescription for 2 weeks with 13.98 g/(kg / d.The model group was fed with isobaric distilled water at the same time, and the normal group had no intervention.The morphological features and body weight of rats were observed, and the number of defecation grains, fecal water content and Bristol classification were compared to evaluate the intestinal sensitivity, intestinal motility, and intestinal mucosal histopathology.Results compared with the normal group, the model group was depressed, irritable, had no obvious change in body weight, significantly increased the number of defecation grains, increased the fecal water content, increased the content of fecal water in the Bristol typing, increased the intestinal sensitivity, and increased the intestinal motility, compared with the model group, and compared with the model group,In Wenshen Jianpi prescription group, the movement was more flexible, occasionally irritable, no significant change in body weight was found, the number of defecation grains decreased, the fecal water content decreased and the Bristol type was transformed to type 4, the intestinal sensitivity and intestinal motility decreased (P0.05).The histopathological manifestations of intestinal mucosa in all groups were not abnormal.Conclusion this physical and chemical stimulation model can be used as an ideal method for the preparation of IBS-D rat model of spleen and kidney yang deficiency syndrome.
【作者单位】：中国中医科学院望京医院脾胃病科;北京中医药大学第一临床医学院;北京市密云中医医院急诊科;北京中医药大学基础医学院;约翰·霍普金斯大学;
【基金】：国家自然科学基金青年基金资助项目(No.81403389)
【分类号】：R259;R-332

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