有氧运动对MI后大鼠心肌组织抑炎因子的影响及调控研究

发布时间：2018-06-17 08:28

  本文选题：有氧运动 + 炎症反应　； 参考：《扬州大学》2017年硕士论文

【摘要】：研究目的:探讨有氧运动对心肌梗死(MI)后大鼠心肌组织抑炎因子白介素-4(IL-4)、白介素-10(IL-10)分泌情况及信号传导蛋白和转录激活物6(STAT6)磷酸化水平的影响,以及STAT6在炎症反应中的作用机制。研究方法:选用30只雄性8周龄的SD大鼠,随机分为假手术组(Sham)、心肌梗死组(MI)、梗死+运动组(ME)。以冠状动脉左前降支(LAD)结扎术构建MI模型,运动方式为间歇有氧运动。运动方案:1周的适应性运动后,10m/min热身10min,然后以20m/min运动8min,再以15 m/min运动2 min,交替4个循环,最后20 m/min运动8min,10 m/min运动2 min后结束。每天训练1次,5 d/w,共训练8 w。以二维超声心动图检测大鼠心脏室壁厚度、射血功能;采用ELISA的方法检测大鼠心肌组织IL-4、IL-10的分泌水平;Western blot检测心肌组织p-STAT6、t-STAT6的表达水平。研究结果:(1)MI手术使大鼠心脏心室壁变薄,射血功能降低,射血分数(EF)与Sham组相比明显减小(P≤0.01);8周有氧运动使MI大鼠的室壁厚度增加、射血功能加强,ME组舒张末期左室后壁厚度(LVPWd)与舒张末期室间隔厚度之和明显高于MI组(P≤0.05),ME组EF比MI组明显升高(P≤0.05)。(2)MI组心重/体重比值(HW/BW)显著低于Sham组(P≤0.05),ME组与MI组相比有明显的增加(P≤0.05);而ME组与MI组肺重/体重比值(LW/BW)相比有明显的降低(P≤0.01)。(3)ME的心肌组织IL-4分泌水平与MI组相比有明显升高(P≤0.05);ME组心肌组织的IL-10分泌水平也显著高于MI组(P≤0.05)。(4)Western blot 结果显示,ME 组的 p-STAT6/t-STAT6 比值显著高于MI 组(P≤0.05)。研究结论:(1)有氧运动有利于MI大鼠心脏舒缩功能的改善;(2)有氧运动有助于MI大鼠心功能的恢复,减轻炎症反应;(3)有氧运动可以升高MI大鼠心肌组织中抑炎因子IL-4、IL-10的分泌水平;(4)有氧运动有助于激活大鼠心肌组织STAT6的磷酸化改变,从而抑制炎症反应。
[Abstract]:Objective: to investigate the effects of aerobic exercise on the secretion of interleukin-4 (IL-4), interleukin-10 (IL-10) and phosphorylation of signal transduction protein and activator 6 (STAT6) in myocardial tissue after myocardial infarction (MI) in rats. And the mechanism of STAT 6 in inflammatory reaction. Methods: thirty male 8-week-old SD rats were randomly divided into sham-operated group, myocardial infarction group and myocardial infarction group. The MI model was established by left anterior descending branch ligation of coronary artery. Exercise program: 1 week of adaptive exercise followed by 10 m / min warm up for 10 minutes, then 20m/min for 8 minutes, then 15 m/min for 2 minutes, alternating 4 cycles, and finally 20 m/min for 8 minutes and 10 m/min for 2 min. Practice once a day for 5 d / w, and train for 8 ws. The myocardial wall thickness and ejection function were measured by two-dimensional echocardiography, the level of IL-4 IL-10 was detected by Elisa and the expression of p-STAT6t-STAT6 was detected by Western blot. Results compared with Sham group, the ventricular wall thinning and ejection function were decreased, and the ventricular wall thickness of MI rats was increased by aerobic exercise for 8 weeks (P 鈮，

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