海水养殖池沉积物中重金属形态和生物酶活性的关系研究

发布时间：2018-06-14 09:01

  本文选题：海水 + 养殖池　； 参考：《海洋与湖沼》2016年01期

【摘要】：以连云港市对虾养殖池表层沉积物为研究对象,采用Tessier连续萃取法,研究了表层沉积物中重金属(Cu、Zn、Pb、Cd、Cr、As、Mn)的赋存形态和酶(脲酶、碱性磷酸酶、过氧化氢酶、转化酶)活性的相关性。结果表明,重金属Cu和Zn以有机-硫化物结合态为主要的赋存形态,Pb以铁锰氧化物态为主要的赋存形态,Cd以可交换态(平均33.47%)和碳酸盐结合态(平均31.16%)为主要存在形态,Cr和As主要以残渣态存在,Mn的铁锰氧化物态比例最高。脲酶的活性范围为0.45—8.96mg/(g·24h),碱性磷酸酶活性范围为2.45—6.69mg/(g·24h),过氧化氢酶活性范围为0.14—2.36m L/(g·min),转化酶活性范围为0.45—10.45mg/(g·24h)。脲酶活性与Cd、As的可交换态、Cd的碳酸盐结合态、Cu、As的铁锰氧化物态、Zn、Pb、Cd、Mn的有机硫化物态之间显著相关;碱性磷酸酶活性与Zn、Cd的铁锰氧化物态显著相关;过氧化氢酶活性与Cd的可交换态、碳酸盐结合态及残渣态、Cu、Cd、As的铁锰氧化物态、Zn、Pb、Cd、Mn的有机硫结合态之间显著相关;转化酶活性与Cu、Cd、As的可交换态、Cd的碳酸盐结合态、Cu、Cd、As的铁锰氧化物态、Zn、Pb、Cd、Mn的有机硫化物态、Cd、As的残渣态之间显著相关。酶活性可以用来指示海水养殖池沉积物中重金属的形态转化过程。
[Abstract]:Taking the surface sediments of shrimp culture ponds in Lianyungang city as the research object, Tessier continuous extraction method was used to study the correlation between the occurrence forms of the heavy metal (Cuznznzn PbPbCdcCrCrAsMnMn) and the activities of urease, alkaline phosphatase, catalase and invertase in the surface sediments of Penaeus penaeus cultured in Lianyungang city. The results show that The main forms of heavy metals Cu and Zn are organic-sulfide-bound forms. The main forms of Pb are iron and manganese oxides. The main forms of CD are exchangeable (33.47) and carbonate bound (31.16). The ratio of iron and manganese oxides with residual mn is the highest. The range of urease activity was 0.45-8.96mg/(g 24 h, alkaline phosphatase activity was 2.45-6.69mg/(g 24 h, catalase activity range was 0.14-2.36 mL / min, invertase activity range was 0.45-10.45mg/(g 24 h. There is a significant correlation between urease activity and the ferromanganese oxide state of the exchangeable cadmium carbonate bound Cucas, the organic sulphide state of Zn PbPbCD CD, the activity of alkaline phosphatase and the ferromanganese oxide state of Zn + CD, and the relation between the activity of urease and the organic sulphide state of Zn ~ (2 +) Pb ~ (2 +) CD ~ (2 +). The activity of catalase is significantly correlated with the exchangeable state of CD, the carbonate-bound state and the Fe-Mn oxide state of the residual form of CuPbCdOAs. There is a significant correlation between invertase activity and the residual state of Cu ~ (2 +) / CD ~ (2 +) / Cu ~ (2 +) / CD ~ (2 +) / Cu ~ (2 +) / CD ~ (2 +) / Cu ~ (2 +) / Cu ~ (2 +) / Cu ~ (2 +) / Cu ~ (2 +) / Cu ~ (2 +) ~ (2 Enzyme activity can be used to indicate the transformation process of heavy metals in the sediments of mariculture ponds.
【作者单位】： 淮海工学院;
【基金】：国家自然科学基金项目,41306114号 江苏省海洋生物技术重点实验室开放项目,2013HS008号 淮海工学院自然科学基金项目,Z2014017号 江苏省海洋科学与技术优势学科建设工程资助项目,5511201401X
【分类号】：X55
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本文编号：2016834