碳量子点的制备分离及其在硝基苯酚同分异构体识别中的应用

发布时间：2018-03-16 19:08

本文选题：碳量子点　切入点：色谱分离　出处：《东北师范大学》2015年硕士论文　论文类型：学位论文

【摘要】：碳量子点(CQDs)作为一类新型荧光纳米材料,近年来受到了广泛的关注和研究。其无毒无环境污染、生物相容性好、易于功能化,且制备方法简单多样、碳源丰富而且价廉,因此应用领域十分宽广。本文在前人工作的基础上,对碳量子点的合成、分离与提纯、应用等做了进一步的研究和探索。主要内容如下:1.以壳聚糖为原料,酸性条件下通过水热法合成了可产生蓝色荧光的碳量子点。用荧光光谱、核磁共振波谱、质谱、紫外-可见吸收光谱、透射电镜、红外光谱等多种先进检测手段对其结构、形貌以及性质进行了表征,并根据质谱,对其形成机理和可能的结构进行了推测。通过细胞溶血实验,对所制备的碳量子点的生物相容性进行了评估。2.利用高效液相色谱对碳量子点进行分离提纯,通过改变固定相、流动相及流速等色谱条件,对碳量子点溶液进行了有效的制备分离,结合质谱分析,进一步推断了制备分离出的碳量子点的可能结构,测定了荧光量子产率。3.将用苯丙氨酸修饰后的碳量子点分别与邻、对硝基苯酚异构体作用,通过考察二异构体对碳量子点荧光强度的影响,探讨了碳量子点应用于硝基苯酚异构体识别的最佳条件。本文的研究表明,利用高效液相色谱分离纯化碳量子点,可以极大地提高碳量子点的荧光量子效率,为解决碳量子点组成复杂、荧光共振明显从而导致荧光量子效率低下并制约碳量子点应用前景的瓶颈问题,进行了有益的尝试。将碳量子点应用于异构体的荧光识别,对开拓碳量子点的应用领域具有积极的作用。
[Abstract]:As a new kind of fluorescent nanomaterials, carbon quantum dots (CQDs) have been widely studied in recent years. They are non-toxic and non-polluting, have good biocompatibility, are easy to be functionalized, and can be prepared by simple and diverse methods, rich in carbon sources and cheap in price. In this paper, the synthesis, separation, purification and application of carbon quantum dots are further studied and explored. The main contents are as follows: 1. Using chitosan as raw material. Carbon quantum dots, which can produce blue fluorescence, were synthesized by hydrothermal method under acidic conditions. Their structures were characterized by fluorescence spectra, NMR spectra, mass spectra, UV-Vis absorption spectra, transmission electron microscopy, infrared spectra, etc. The morphology and properties were characterized, and its formation mechanism and possible structure were inferred by mass spectrometry. The biocompatibility of the prepared carbon quantum dots was evaluated. 2. The separation and purification of carbon quantum dots were carried out by high performance liquid chromatography (HPLC), and the chromatographic conditions such as stationary phase, mobile phase and flow rate were changed. The effective preparation and separation of carbon quantum dot solution were carried out. The possible structure of the carbon quantum dot was further inferred by mass spectrometry, and the fluorescence quantum yield was determined. 3. The carbon quantum dot modified with phenylalanine was separated from adjacent carbon quantum dot. The effects of diisomers on the fluorescence intensity of carbon quantum dots (QDs) were investigated, and the optimum conditions for the application of QDs to the recognition of nitrophenol isomers were discussed. The fluorescence quantum efficiency of carbon quantum dots can be greatly improved by the separation and purification of carbon quantum dots by high performance liquid chromatography. The fluorescence resonance obviously leads to the low fluorescence quantum efficiency and restricts the application prospect of carbon quantum dots, and a useful attempt has been made to apply carbon quantum dots to the fluorescence recognition of isomers. It plays an active role in developing the application field of carbon quantum dots.
【学位授予单位】：东北师范大学
【学位级别】：硕士
【学位授予年份】：2015
【分类号】：TQ127.11;O657

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