东海海域表层沉积物中硫酸盐还原菌分布特征研究
发布时间:2018-05-12 15:11
本文选题:东海 + 硫酸盐还原菌 ; 参考:《中国环境科学》2016年12期
【摘要】:利用2011年4、7、8和10月对东海海域4个航次的调查资料,以表层沉积物中硫酸盐还原菌(SRB)为研究对象,针对于SRB所共有的异化型亚硫酸盐还原酶(DSR)中的β亚基基因(dsr B),通过荧光定量PCR技术对SRB丰度的时空分布特征进行了描述.结果表明,SRB丰度变化范围为1.87×10~5~4.69×10~8cells/g,平均值为1.15×10~8cells/g,且4月SRB丰度最低,7月SRB丰度最高;SRB数量在总细菌中的比例介于0.0039%~1.6176%之间,说明SRB在东海表层沉积物的细菌总量中比例很小;SRB丰度的水平分布特征整体表现为南部海域高于北部海域,长江口及浙闽沿岸泥质区高于非泥质区.此外,SRB丰度与环境因子的相关性分析表明,温度和溶解氧是影响SRB丰度的重要因素.
[Abstract]:Based on the survey data of 4 voyages in the East China Sea in April 2011 and October 2011, the sulfate reducing bacteria SRB in surface sediments was used as the research object. The spatial and temporal distribution of SRB abundance was described by fluorescence quantitative PCR for the 尾 -subunit gene (DSR) of dissimilated sulfite reductase (DSRs) shared by SRB. The results showed that SRB abundance varied from 1.87 脳 10 ~ (5) to 4.69 脳 10 ~ (8) cells / g with an average value of 1.15 脳 10 ~ (8) cells / g, and the lowest abundance of SRB was observed in April, and the ratio of SRB abundance to total bacteria in July was between 0.0039 and 1.6176%. It is concluded that the horizontal distribution of SRB abundance in the surface sediments of the East China Sea is higher in the southern sea than in the north, and in the muddy areas along the Yangtze Estuary and Zhejiang and Fujian coasts is higher than that in the non-muddy areas. In addition, the correlation analysis between SRB abundance and environmental factors showed that temperature and dissolved oxygen were important factors affecting SRB abundance.
【作者单位】: 中国海洋大学环境科学与工程学院;海洋环境与生态教育部重点实验室;海洋国家实验室海洋生态与环境科学功能实验室;中国海洋大学海洋生命学院;
【基金】:国家自然科学基金(41521064;41620104001) 中国科学院海洋生态与环境科学重点实验室 青岛海洋科学与技术国家实验室海洋生态与环境科学开放基金(KLMEES201601)
【分类号】:X172
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