以预处理污泥为底物生产微生物絮凝剂的研究
发布时间：2018-08-15 17:24
【摘要】：生物絮凝剂因其自身优点及瓶颈问题,使得它在污水净化生产实践一直受到广泛关注,而培养产絮菌的基质价格贵问题是妨碍絮凝剂大规模应用于净化污水和工业生产的最重要原因。本课题就絮凝剂产生菌发酵培养成本较高问题提出新的思路,以污泥处理后的提取液作为原料制备污泥提取液发酵培养基,对发酵过程中的环境因子进行考察,最后对这一研究进行了综合效益评估。对污泥进行不同条件(不同温度、时间、酸碱度)的预处理,分析其提取液的TOC浓度、TN浓度、TP浓度等,从而确定最佳的预处理条件。絮凝剂产生菌LLin6直接利用污泥提取液发酵产絮效果并不理想,经过调节浓度和补加物质,确定20~25 g/L的污泥在p H=12条件下处理3 h,后经离心去除沉淀留取上清液备用,调节p H值至7.0左右,添加8 g/L的葡萄糖,112℃灭菌30 min后接入絮凝剂产生菌LLin6进行发酵培养,24 h发酵后可以获得85%以上的絮凝率。对絮凝剂产生菌LLin6在所配制的污泥提取液培养基中生长状况进行分析发现,产絮菌LLin6在污泥提取液培养基中生长状况良好。以污泥提取液作为培养基原料,对絮凝剂产生菌LLin6在其中产絮发酵进行考察。在四组单因素实验基础上,利用响应曲面法预测絮凝剂产生菌LLin6在污泥提取液中最佳发酵条件:发酵温度24.7℃、培养基初始p H为6.5、接种量为6%、发酵时间22 h,此条件下理论上可以获得的絮凝率为87.14%。而实际絮凝率为85.54%,与预测值相接近,证明响应曲面分析法得到的发酵条件可信度高,可以预测响应值。提出产絮菌LLin6以污泥提取液为底物生产微生物絮凝剂的工艺流程,对以处理后的剩余污泥为底物制备生物絮凝剂的产品进行了成本分析,产3.5T液体生物絮凝剂,可资源化利用剩余污泥(浓度一般为6 g/L)约14.6 T,生物絮凝剂原料成本由730元降低了404.5元,相当节约了的55%以上,大幅降低了原料成本。除了经济效益,还有它随之带来的社会和环境效益。
[Abstract]:Because of its advantages and bottlenecks, bioflocculants have been widely concerned in the production of sewage purification. The most important reason for the large-scale application of flocculant in wastewater purification and industrial production is the expensive substrate price of flocculant. In this paper, a new idea about the high cost of flocculant-producing bacteria fermentation was put forward. The sludge extract was used as the raw material to prepare the fermentation medium of sludge extract, and the environmental factors in the process of fermentation were investigated. Finally, the comprehensive benefit evaluation of this study is carried out. The sludge was pretreated with different conditions (different temperature, time, pH), and the TOC concentration, TN concentration and TP concentration of the extract were analyzed, so as to determine the best pretreatment conditions. The flocculant producing strain LLin6 directly used sludge extract to ferment flocculating effect is not ideal. After adjusting the concentration and adding substance, the sludge of 20 ~ 25 g / L was treated at pH 12 for 3 h, and then centrifugally removed and precipitated to take the supernatant. After adjusting pH value to 7.0, adding 8 g / L glucose at 112 鈩，
本文编号：2184899
[Abstract]:Because of its advantages and bottlenecks, bioflocculants have been widely concerned in the production of sewage purification. The most important reason for the large-scale application of flocculant in wastewater purification and industrial production is the expensive substrate price of flocculant. In this paper, a new idea about the high cost of flocculant-producing bacteria fermentation was put forward. The sludge extract was used as the raw material to prepare the fermentation medium of sludge extract, and the environmental factors in the process of fermentation were investigated. Finally, the comprehensive benefit evaluation of this study is carried out. The sludge was pretreated with different conditions (different temperature, time, pH), and the TOC concentration, TN concentration and TP concentration of the extract were analyzed, so as to determine the best pretreatment conditions. The flocculant producing strain LLin6 directly used sludge extract to ferment flocculating effect is not ideal. After adjusting the concentration and adding substance, the sludge of 20 ~ 25 g / L was treated at pH 12 for 3 h, and then centrifugally removed and precipitated to take the supernatant. After adjusting pH value to 7.0, adding 8 g / L glucose at 112 鈩，
本文编号：2184899
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