2株番木瓜根际促生菌的解磷解钾作用

发布时间：2018-03-17 03:23

本文选题：番木瓜　切入点：根际促生菌　出处：《江苏农业科学》2017年06期 　论文类型：期刊论文

【摘要】：用平板涂布、划线与固体平板-透明圈法从番木瓜根际土壤中筛选出2株解磷解钾细菌(PK-1、PK-2)。通过摇瓶培养,测定PK-1、PK-2发酵液中水溶性磷、水溶性钾含量和磷、钾总量;分析PK-1、PK-2解磷解钾能力;对PK-1、PK-2进行分子鉴定。结果表明,PK-1溶解无机磷能力的溶量为1.63μg/m L,PK-2溶量为17.26μg/m L,溶解无机磷能力表现为PK-1㩳PK-2,即PK-2具有更强的解无机磷能力;PK-1溶解有机磷能力的溶量为8.49μg/m L,PK-2溶量为2.38μg/m L,溶解有机磷能力表现为PK-1PK-2,即PK-1的解有机磷能力更好;PK-1溶解钾能力的溶量为8.15μg/m L,PK-2溶量为8.49μg/m L,溶解钾能力表现为PK-1㩳PK-2,PK-1和PK-2的解钾能力接近。经16S r DNA序列分析,初步鉴定PK-1为肠杆菌属(Enterobacter),PK-2为芽孢杆菌属(Bacillus)。供试菌株PK-1和PK-2具有良好的解磷解钾功能,可为进一步研制专用的番木瓜微生物肥料提供菌种资源和依据。
[Abstract]:Two strains of PK-1PK-2K bacteria were screened from papaya rhizosphere soil by plate coating, streaking and solid plate-transparent circle method. The soluble phosphorus, water-soluble potassium and the total amount of phosphorus and potassium in the fermentation broth of PK-1 and PK-2 were determined by shaking flask culture. The results show that the solubility of PK-1 is 1.63 渭 g / m ~ (-1) / m ~ (-1) PK-2 is 17.26 渭 g / m ~ (-1), and the ability of dissolving inorganic phosphorus is PK-1 ~ (-1)? PK-2, that is, PK-2 has a stronger ability to dissolve inorganic phosphorus. The solubility of PK-1 is 8.49 渭 g / mL PK-2 is 2.38 渭 g / mL, and the ability of dissolving organic phosphorus is PK-1PK-2. That is, the ability of PK-1 to dissolve organophosphorus is better than that of PK-1. The solubility of PK-1 is 8.15 渭 g / m LL-PK-2. 8.49 渭 g / m L, the ability to dissolve potassium is PK-1? By 16s r DNA sequence analysis, PK-1 was identified as Enterobacter tumefaciens PK-2 as Bacillus spp. The tested strains PK-1 and PK-2 had good phosphorolytic activity. It can provide strain resources and basis for further development of special microbial fertilizer for papaya.
【作者单位】：西南林业大学国家林业局西南地区生物多样性保育重点实验室;广西壮族自治区亚热带作物研究所;
【基金】：云南省森林灾害预警与控制重点实验室开放基金(编号:ZK14A101) 云南省教育厅科学研究基金(编号:515006017) 西南林业大学林学一级学科博士研究生创新基金
【分类号】：S154.3;S667.9

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