胶孢炭疽菌CgSho1基因的克隆与功能分析

发布时间：2018-04-14 05:35

  本文选题：胶胞炭疽菌 + MAPK途径　； 参考：《植物病理学报》2017年01期

【摘要】：HOG-MAPK(high osmolarity glycerol mitogen-activated protein kinase)信号途径是真菌MAPK途径中参与渗透压响应的一条重要通路,在植物病原菌生长发育及致病过程中发挥着重要的作用。Sho1(synthetic high osmolarity-sensitive protein1)是HOG-M APK信号途径上游的一个重要感受器,在不同真菌中常具有不同的功能。本研究从胶孢炭疽菌中克隆了Sho1的同源基因,命名为Cg Sho1,该基因编码一个291个氨基酸的蛋白,含有4个跨膜结构域和一个SH3功能域。利用同源重组的方法获得了该基因的敲除突变体,与野生型相比,敲除突变体表现为营养生长缓慢,菌丝稀疏且疏水性增强,产孢量下降,对氧化压力和渗透压更加敏感,致病力明显减弱。上述结果表明,Cg Sho1参与调控胶胞炭疽菌的营养生长、分生孢子产量、氧化应激反应、渗透压响应及致病性。
[Abstract]:The HOG-MAPK(high osmolarity glycerol mitogen-activated protein kinase pathway is an important pathway involved in osmotic pressure response in the MAPK pathway of fungi.Sho1synthetic high osmolarity-sensitive protein 1 is an important receptor upstream of HOG-M APK signaling pathway and has different functions in different fungi.In this study, the homologous gene of Sho1 was cloned from Bacillus anthracis, named CG Sho1, which encodes a 291 amino acid protein containing four transmembrane domains and one SH3 functional domain.The knockout mutants of the gene were obtained by homologous recombination. Compared with the wild type, the knockout mutants showed slow vegetative growth, sparse hypha and enhanced hydrophobicity, decreased sporulation, and were more sensitive to oxidation pressure and osmotic pressure.The pathogenicity was obviously weakened.The results indicated that CG Sho1 was involved in the regulation of the vegetative growth, conidial yield, oxidative stress response, osmotic pressure response and pathogenicity of Colletotrichum anthracis.
【作者单位】： 海南大学环境与植物保护学院;
【基金】：国家自然科学基金(31560045) 海南省自然科学基金(20153132)
【分类号】：S432.44
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本文编号：1747959