芬太尼中毒死亡大白兔体内分布研究

发布时间：2018-08-07 17:32

【摘要】：目的建立芬太尼中毒致死的动物模型,探讨芬太尼在致死大白兔体内的分布规律。方法用6只雄性大白兔按5.4mg/kg(2LD50)经耳缘静脉推注芬太尼注射液,大白兔死后迅速解剖并提取心、肝、脾、肺、肾、脑、肌肉、睾丸、胃、心血、周围血、胆汁和尿液,用正己烷∶乙醇(20∶1)萃取,利用UPLC-MSn法检测各组织和体液中芬太尼含量,使用SPSS15.0进行方差分析,均数两两比较的SNK法进行统计分析。检验水准为α=0.05。结果实验大白兔给药后1min出现颈项强直、四肢抽搐等中毒症状,平均4.7min因呼吸抑制而死亡。死后肺内芬太尼含量最高,其次是肾和心,而尿液中含量较低。结论本实验的结果与相关案例报道基本吻合,提示肺、肾和心脏是芬太尼中毒案件鉴定的理想检材,芬太尼在致死大白兔体内的分布规律可为相关案件的鉴定提供一定的依据。
[Abstract]:Objective to establish an animal model of fentanyl poisoning and to investigate the distribution of fentanyl in rabbits. Methods Fentanyl injection was injected into 6 male rabbits via 5.4mg/kg (2LD50) via auricular vein. The heart, liver, spleen, lung, kidney, brain, muscle, testis, stomach, heart blood, peripheral blood, bile and urine were rapidly dissected and extracted after death. The content of fentanyl in tissues and body fluids was determined by UPLC-MSn method with extraction of n-hexane and ethanol (20:1). The variance analysis was performed by SPSS15.0 and the statistical analysis was carried out by SNK method. The test level is 伪 0. 05. Results after administration of the drug, 1min appeared cervical ankylosis, limb convulsion and other toxic symptoms in rabbits. The average 4.7min died because of respiratory inhibition. Postmortem lung fentanyl was the highest, followed by kidney and heart, while urine was lower. Conclusion the results of this experiment are basically consistent with the reports of related cases, suggesting that lung, kidney and heart are ideal samples for identification of fentanyl poisoning cases, and the distribution of fentanyl in the body of lethal rabbits can provide some basis for the identification of related cases.
【作者单位】：中国人民公安大学;北京市公安局法医检验鉴定中心;山西医科大学法医学院;公安部物证鉴定中心;
【基金】：公安部重点研究计划项目(2009ZDYJBJSJ001)
【分类号】：D919

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