大鼠皮肤切创愈合过程中Ubiquitin的表达及其法医学意义

发布时间：2019-02-13 01:47

【摘要】： 目的:泛素(Ubiquitin)是热休克蛋白家族中分子量最小的一员,在真核生物中广泛存在,其分布非常丰富,从生殖细胞到各种体细胞都广泛存在。它由76个氨基酸组成,分子量约8.5KD,其氨基酸序列高度保守。在应激因素作用下,细胞内形成各种异常蛋白质,对细胞造成严重损伤,此时,Ubiquitin将会与之共价结合,再通过蛋白酶体将其降解,以恢复细胞的正常功能。这种蛋白质与Ubiquitin结合的现象称为蛋白质泛素化,细胞内蛋白质泛素化参与了细胞的多种生理过程。近年来的研究表明,在刺激、损伤等多种因素的作用下,Ubiquitin可被诱导产生,它与阿茨海默氏病、肿瘤等多种疾病或损伤有密切关系。目前,Ubiquitin在法医学领域的研究在世界范围内都显得比较匮乏,尤其是对Ubiquitin与皮肤损伤以及由此产生的损伤时间推断问题的研究更是几乎一片空白。本实验建立大鼠皮肤切创模型,对Ubiquitin在皮肤创伤愈合过程中的表达情况和表达规律进行研究,探讨其在皮肤损伤中的作用及机制,为解决损伤时间推断这一法医学难题提供新的方向和实验室依据。方法:采用完全随机分组方法,根据造创后处死时间不同将实验大鼠随机分为伤后1h、3h、6h、12h、1d、3d、6d、10d、14d组,另设
[Abstract]:Objective: ubiquitin (Ubiquitin) is the smallest member of the heat shock protein family. It is widely distributed in eukaryotes, ranging from germ cells to various somatic cells. It is composed of 76 amino acids and its molecular weight is about 8.5 KD, and its amino acid sequence is highly conserved. Under the action of stress factors, various abnormal proteins were formed in the cells, which caused serious damage to the cells. At this time, Ubiquitin would bind to it covalently, and then degrade it through proteasome, so as to restore the normal function of the cells. This phenomenon of protein binding to Ubiquitin is called protein ubiquification, which is involved in many physiological processes. Recent studies have shown that Ubiquitin can be induced by stimulation, injury and other factors, and it is closely related to many diseases or injuries such as Alzheimer's disease, tumor and so on. At present, the research of Ubiquitin in the field of forensic medicine is relatively scarce in the world, especially the research on Ubiquitin and skin injury and the estimation of injury time caused by it is almost a blank. In this study, a rat skin injury model was established to investigate the expression and regularity of Ubiquitin in the process of skin wound healing, and to explore the role and mechanism of Ubiquitin in skin injury. It provides a new direction and laboratory basis for solving the forensic medical problem of estimating injury time. Methods: the experimental rats were randomly divided into 4 groups according to the time of death after trauma. The rats were randomly divided into 1 hour, 3 h, 12 h, 1 day, 1 day, 3 d, 6 d, 10 d and 14 d groups, and the experimental rats were randomly divided into 4 groups.
【学位授予单位】：重庆医科大学
【学位级别】：硕士
【学位授予年份】：2006
【分类号】：D919

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