三种典型淡水藻EOM组成特征及胞外氨基酸释放规律

发布时间：2018-02-16 02:17

本文关键词： 有机氮胞外有机物氨基酸贡献　出处：《中国环境科学》2017年05期 　论文类型：期刊论文

【摘要】：通过对比分析了藻类EOM的类型和组成,探讨了藻类胞外氨基酸的释放规律.选取典型淡水藻铜绿微囊藻(Microcystis aeruginosa)、四尾栅藻(Scenedesmus quadricauda)、喙头舟形藻(Navicula rhynchocephala)为研究对象,采用三维荧光光谱-平行因子分析法(EEMs-PARAFAC)、柱前衍生高效液相色谱(HPLC)分析3种藻在生长稳定期EOM特征与胞外游离氨基酸、结合氨基酸的含量组成等差异.结果表明,藻类均可将无机氮转化为有机氮释放到水体中;3种藻EOM中总色氨酸与总酪氨酸荧光强度关系分别为铜绿微囊藻四尾栅藻喙头舟形藻、喙头舟形藻四尾栅藻铜绿微囊藻;藻胞外氨基酸中组氨酸、丙氨酸、甘氨酸及丝氨酸含量均较高,苏氨酸、精氨酸、酪氨酸含量均较低;3种藻c(DFAAs)/c(DCAAs)的比值均较低,释放的氨基酸多数以蛋白质和多肽的形式存在;铜绿微囊藻胞外有机物(EOM)中ρ(DON)、ρ(TDAAs)及氨基酸对水体中DON贡献量均为最高,分别为1.31mg/L、5.35mg/L、69.08%,因此蓝藻水华暴发可能会增加水体的氨基酸浓度,加重水体有机氮负荷.
[Abstract]:By comparing and analyzing the types and composition of algae EOM, the release regularity of extracellular amino acids of algae was studied. Microcystis aeruginosaer, Scenedesmus quadricauda and Navicula rhynchocephala were selected as the research objects. The EOM characteristics and extracellular free amino acids of three species of algae in stable growth period were analyzed by means of three dimensional fluorescence spectroscopic parallel factor analysis (EEMs-PARAFAC) and precolumn derivatization high performance liquid chromatography (HPLC). The relationship between total tryptophan and total tyrosine fluorescence intensity in EOM of three species of algae were respectively: Microcystis aeruginosa, Cyclophora aeruginosa, P. aeruginosa, P. aeruginosa, P. aeruginosa, P. aeruginosa, and P. aeruginosa, respectively, the relationship between total tryptophan and total tyrosine fluorescence intensity was: Microcystis aeruginosa; The contents of histidine, alanine, glycine and serine were higher in the extracellular amino acids of alginate. The contents of threonine, arginine and tyrosine were all lower. In the extracellular organic matter of Microcystis aeruginosa (EOM), the contribution of 蟻 ~ (2 +) ~ (2 +) and amino acid to DON in water was the highest (1.31 mg / L ~ (5.35) mg / L ~ (-1)), respectively. Therefore, the outbreak of Shui Hua may increase the concentration of amino acid and increase the load of organic nitrogen in water.
【作者单位】：南昌大学资源环境与化工学院;环境基准与风险评估国家重点实验室;湖泊水污染治理与生态修复技术国家工程实验室;
【基金】：国家水体污染控制与治理科技重大专项(2012ZX07102-004)
【分类号】：X52

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