纳米纤维基比色生物传感器的制备及其抗生素检测应用
发布时间：2025-04-18 03:07
抗生素因具有选择性抑制或杀灭其它菌种微生物的能力,而被广泛应用于医疗、农业及畜牧养殖业,尤其是随着集约化畜牧业的发展,使用抗生素己成为保障畜牧业持续健康发展所必不可少的核心环节。由于科学知识的缺乏和经济利益的驱使,养殖业成为了四环素、氨基糖苷等各类兽用抗生素滥用的重灾区。抗生素滥用的直接后果是导致其在动物源性食品中的残留,后经食物链在人体内蓄积,引发过敏和变态反应、损伤神经系统、抑制骨髓造血机能、导致耐药菌株感染等危害。当前,抗生素耐药性细菌已蔓延至全球各地,如不采取行动对滥用进行干预,人类将“无药可救”。世界卫生组织也将抗生素滥用视为国际关注的重大问题之一,对相关重点抗生素的每日摄入量（Acceptable daily intake,ADI）和最大残留限量（Maximum residue limit,MRL）做出了规定,以期管控动物源性食品及环境中的抗生素残留。现有的抗生素检测方法有高效液相色谱、毛细管电泳法、电化学分析仪及微生物分析法等,尽管上述方法灵敏度高、选择性好且检出限低,但也面临仪器昂贵、检测成本高、操作繁琐及测定时间长等不足,无法充分满足大批样品的现场过筛需求。因此,研发灵...
【文章页数】：267 页
【学位级别】：博士
【文章目录】：
ACKNOWLEDGEMENTS
DEDICATION
ABSTRACT
摘要
ABBREVIATION
CHAPTER1.INTRODUCTION AND LITERATURE REVIEW
1.1.Introduction
1.2.Overview of antibiotics
1.2.1.Classification,structure,and application
1.2.2.Status and hazards of antibiotics residues
1.2.3.Measures to prevent antibiotics residues
1.3.Traditional techniques for the detection of antibiotic residues
1.3.1.HPLC
1.3.2.Electrochemical analysis for antibiotics detection
1.3.3.Ultraviolet(UV)detection methods
1.3.4.Mass spectrometry(MS)
1.3.5.Comparison of traditional antibiotic detection methods
1.4.Colorimetric analysis and its application
1.4.1.Overview of colorimetric analysis
1.4.2.The basis of the colorimetric method
1.4.3.Application of the colorimetric method in antibiotic detection
1.5.Electrospun nanofibers and their application in sensing
1.5.1.Introduction to electrospinning
1.5.2.Nanostructure by electrospinning
1.5.3.Application of electrospinning nanofibers in the sensor field
1.6.Purpose and objective of the study
1.7.Innovation points
1.8.Limitation
1.9.Background and research contents of the thesis
1.10.References
CHAPTER2.OXYTETRACYCLINE STRIPS BASED ON NICKEL(II)IONS IMMOBILIZED NANOFIBROUS MEMBRANES
2.1.Introduction
2.2.Experimental
2.2.1.Materials and reagents
2.2.2.Preparation of PAN NFMs
2.2.3.Surface modification of PAN NFMs
2.2.4.Fabrication of colorimetric strips
2.2.5.Detection of OTC
2.2.6.Characterization
2.3.Results and discussion
2.3.1.Fabrication of colorimetric strips
2.3.2.The optimization of detection conditions
2.3.3.The sensitivity of colorimetric strips
2.3.4.Selectivity and reversibility of colorimetric strips
2.4.Chapter summary
2.5.References
CHAPTER3.TETRACYCLINE STRIPS BASED ON IRON(III)AND COPPER(II)IMPREGNATED IMMOBILIZED NANOFIBROUS MEMBRANES
3.1.Introduction
3.2.Experimental
3.2.1.Chemicals and materials
3.2.2.Preparation of Fe@alginate/PAN nanofibers
3.2.3.Preparation of Cu@EPAN nanofibers
3.2.4.Characterization of TCs sensing performance
3.2.5.Characterization
3.3.Results and discussion
3.3.1.Fe@alginate/PAN nanofibers
3.3.2.Cu@EPAN nanofibers
3.4.Chapter summary
3.5.References
CHAPTER4.METRONIDAZOLE NON-ENZYMATIC COLORIMETRIC SENSORS STRIP BASED ON MELAMINE-FUNCTIONALIZED GOLD NANOPARTICLES ASSEMBLED POLYAMIDE NANOFIBERS MEMBRANES
4.1.Introduction
4.2.Experimental
4.2.1.Chemicals and materials
4.2.2.Preparation and functionalization of gold nanoparticles
4.2.3.Preparation of the PA6 NFMs
4.2.4.Preparation of colorimetric strips and sensing experiment
4.2.5.Real samples preparation
4.2.6.Characterization
4.3.Results and discussion
4.3.1.Sensing concept
4.3.2.Characteristics of AuNPs and MA@AuNPs
4.3.3.Characterization of MA@AuNPs immobilized PA6 NFMs
4.3.4.Optimization of detection conditions
4.3.5.The detection performance of strips
4.4.Chapter summary
4.5.References
CHAPTER5.KANAMYCIN COLORIMETRIC STRIPS BASED ON APTAMER-GOLD NANOPARTICLE IMMOBILIZED NANOFIBROUS MEMBRANES
5.1.Introduction
5.2.Experimental
5.2.1.Chemicals and materials
5.2.2.Preparation and bioconjugation of gold nanoparticles
5.2.3.G-CA NFMs fabrication
5.2.4.Apt@Au assembled G-CA NFMs and experimented for KMC detection
5.2.5.Real samples preparation
5.2.6.Characterization
5.3.Results and discussion
5.3.1.Design and principle of the proposed biosensing
5.3.2.Apt@Au probes characterization
5.3.3.Characterization of GA grafted CA NFMs
5.3.4.Characterization of Apt@Au assembled GA grafted CA NFMs
5.3.5.Biosensing performance analysis
5.4.Chapter summary
5.5.References
CHAPTER6.CONCLUSIONS AND RECOMMENDATIONS
6.1.Conclusions
6.2.Future work and recommendations
LIST OF PUBLICATIONS
APPENDIX A
本文编号：4040426
【文章页数】：267 页
【学位级别】：博士
【文章目录】：
ACKNOWLEDGEMENTS
DEDICATION
ABSTRACT
摘要
ABBREVIATION
CHAPTER1.INTRODUCTION AND LITERATURE REVIEW
1.1.Introduction
1.2.Overview of antibiotics
1.2.1.Classification,structure,and application
1.2.2.Status and hazards of antibiotics residues
1.2.3.Measures to prevent antibiotics residues
1.3.Traditional techniques for the detection of antibiotic residues
1.3.1.HPLC
1.3.2.Electrochemical analysis for antibiotics detection
1.3.3.Ultraviolet(UV)detection methods
1.3.4.Mass spectrometry(MS)
1.3.5.Comparison of traditional antibiotic detection methods
1.4.Colorimetric analysis and its application
1.4.1.Overview of colorimetric analysis
1.4.2.The basis of the colorimetric method
1.4.3.Application of the colorimetric method in antibiotic detection
1.5.Electrospun nanofibers and their application in sensing
1.5.1.Introduction to electrospinning
1.5.2.Nanostructure by electrospinning
1.5.3.Application of electrospinning nanofibers in the sensor field
1.6.Purpose and objective of the study
1.7.Innovation points
1.8.Limitation
1.9.Background and research contents of the thesis
1.10.References
CHAPTER2.OXYTETRACYCLINE STRIPS BASED ON NICKEL(II)IONS IMMOBILIZED NANOFIBROUS MEMBRANES
2.1.Introduction
2.2.Experimental
2.2.1.Materials and reagents
2.2.2.Preparation of PAN NFMs
2.2.3.Surface modification of PAN NFMs
2.2.4.Fabrication of colorimetric strips
2.2.5.Detection of OTC
2.2.6.Characterization
2.3.Results and discussion
2.3.1.Fabrication of colorimetric strips
2.3.2.The optimization of detection conditions
2.3.3.The sensitivity of colorimetric strips
2.3.4.Selectivity and reversibility of colorimetric strips
2.4.Chapter summary
2.5.References
CHAPTER3.TETRACYCLINE STRIPS BASED ON IRON(III)AND COPPER(II)IMPREGNATED IMMOBILIZED NANOFIBROUS MEMBRANES
3.1.Introduction
3.2.Experimental
3.2.1.Chemicals and materials
3.2.2.Preparation of Fe@alginate/PAN nanofibers
3.2.3.Preparation of Cu@EPAN nanofibers
3.2.4.Characterization of TCs sensing performance
3.2.5.Characterization
3.3.Results and discussion
3.3.1.Fe@alginate/PAN nanofibers
3.3.2.Cu@EPAN nanofibers
3.4.Chapter summary
3.5.References
CHAPTER4.METRONIDAZOLE NON-ENZYMATIC COLORIMETRIC SENSORS STRIP BASED ON MELAMINE-FUNCTIONALIZED GOLD NANOPARTICLES ASSEMBLED POLYAMIDE NANOFIBERS MEMBRANES
4.1.Introduction
4.2.Experimental
4.2.1.Chemicals and materials
4.2.2.Preparation and functionalization of gold nanoparticles
4.2.3.Preparation of the PA6 NFMs
4.2.4.Preparation of colorimetric strips and sensing experiment
4.2.5.Real samples preparation
4.2.6.Characterization
4.3.Results and discussion
4.3.1.Sensing concept
4.3.2.Characteristics of AuNPs and MA@AuNPs
4.3.3.Characterization of MA@AuNPs immobilized PA6 NFMs
4.3.4.Optimization of detection conditions
4.3.5.The detection performance of strips
4.4.Chapter summary
4.5.References
CHAPTER5.KANAMYCIN COLORIMETRIC STRIPS BASED ON APTAMER-GOLD NANOPARTICLE IMMOBILIZED NANOFIBROUS MEMBRANES
5.1.Introduction
5.2.Experimental
5.2.1.Chemicals and materials
5.2.2.Preparation and bioconjugation of gold nanoparticles
5.2.3.G-CA NFMs fabrication
5.2.4.Apt@Au assembled G-CA NFMs and experimented for KMC detection
5.2.5.Real samples preparation
5.2.6.Characterization
5.3.Results and discussion
5.3.1.Design and principle of the proposed biosensing
5.3.2.Apt@Au probes characterization
5.3.3.Characterization of GA grafted CA NFMs
5.3.4.Characterization of Apt@Au assembled GA grafted CA NFMs
5.3.5.Biosensing performance analysis
5.4.Chapter summary
5.5.References
CHAPTER6.CONCLUSIONS AND RECOMMENDATIONS
6.1.Conclusions
6.2.Future work and recommendations
LIST OF PUBLICATIONS
APPENDIX A
本文编号：4040426
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