犬细小病毒病例生化指标的研究

发布时间：2018-03-02 14:41

本文选题：犬细小病毒　切入点：血液生化　出处：《河南科技大学》2017年硕士论文　论文类型：学位论文

【摘要】：犬细小病毒病(Canine parvovirus,CPV)多发于幼犬和未接种疫苗的犬,死亡率一直都很高。CPV病例多表现呕吐,腹泻,便血等症状,在临床中多为对症治疗,而对CPV发病时期器官功能学研究较少。本课题通过对CPV患犬在治疗中血液生化指标进行连续监测,对其进行分析处理来研究CPV患犬的血液生化指标的变化情况。并找出与CPV患犬密切相关的生化指标,弄清CPV患犬生化指标的变化及意义,阐明其相关机制,为CPV临床治疗提供指导,进而提高CPV的治愈率。CPV患犬的处理:对符合试验条件的CPV患犬进行3次血液生化指标检测,分别是在患犬入院当天,入院治疗的第三天及第五天进行,检测在当天用药治疗开始前进行。数据的处理:指标样本测定的结果不在参考范围内时,计为变异1次,得出变异样本总数,将变异样本总数除以指标样本总数,即为该指标变异样本率;把生化各项指标划分为六项功能,对每项功能包含指标的变异样本做求和处理,即为变异样本总数,与该功能包含指标的总样本数相比,即为该功能变异百分率;CPV痊愈犬与死亡犬生化指标的对比:试验分痊愈、死亡、对照三组,分析三组实验结果。CPV连续病例的监测:选取在CPV病例中变化明显的五项生化指标,淀粉酶、脂肪酶、肌酸激酶、碱性磷酸酶、白蛋白,在完成三次监测的死亡病例和存活病例中,对比分析两组结果。CPV病例生化指标样本变异率变化比较明显的是:碱性磷酸酶、肌酸激酶、血清钙、天门冬氨酸氨基转移酶、总胆红素、白蛋白、脂肪酶、血清磷,CPV对患犬生化指标影响情况为:碱性磷酸酶浓度升高,肌酸激酶浓度升高,血清钙浓度降低,天门冬氨酸氨基转移酶浓度升高,总胆红素浓度降低,白蛋白浓度降低,脂肪酶浓度升高,血清磷浓度升高;CPV病例显著性变化的脏器(指标)有心脏功能、血清钙、肝功能、白蛋白和胰腺功能;对痊愈组与死亡组进行对比结果显示,有显著性差异的是胰腺功能降低、血清钙浓度下降及白蛋白缺乏,这可能是导致CPV患犬最终死亡的主要原因;在CPV指标的连续检测过程中,指标的变化情况可能预示着疾病发展的方向。结果表明,CPV患犬在治疗期间,应及时监测碱性磷酸酶、肌酸激酶、血清钙、天门冬氨酸氨基转移酶、总胆红素、白蛋白、脂肪酶、血清磷等生化指标,特别是胰腺功能、血清钙及蛋白质指标的变化,根据检测结果,随时调整临床用药,可能会提高CPV的治疗率,降低死亡率。
[Abstract]:Canine parvovirus virus (CPV) is more common in puppies and unvaccinated dogs. The mortality rate of CPV cases is always very high. CPV cases often show vomiting, diarrhea, hematochezia and other symptoms. However, there were few studies on organ function during the onset of CPV. The aim of this study was to continuously monitor the blood biochemical indexes of CPV dogs during the course of treatment. To study the changes of blood biochemical indexes in dogs with CPV, to find out the biochemical indexes closely related to CPV dogs, to find out the changes and significance of biochemical indexes of CPV dogs, and to elucidate the related mechanism. To provide guidance for the clinical treatment of CPV, and then to improve the cure rate of CPV. The treatment of CPV. The blood biochemical indexes of CPV dogs that meet the test conditions were tested three times, respectively, on the day of admission, on the third and fifth day of admission, respectively. Data processing: when the results of the index sample determination are not within the reference range, they are counted as one variation, the total number of the variation samples is obtained, and the total number of the variation samples is divided by the total number of the index samples. That is to say, the sample rate of variation is the index, the biochemical indexes are divided into six functions, and the sum of the variation samples that each function contains, that is, the total number of variation samples, is compared with the total sample number of the index included by the function. That is to say, the percentage of functional variation and the comparison of biochemical indexes between CPV cured dogs and dead dogs were compared. The results of the three groups were analyzed as follows: the monitoring of continuous cases of CPV: selecting five biochemical indexes which changed obviously in CPV cases. Amylase, Lipase, Creatine Kinase, Alkaline Phosphatase, Albumin. In the death and survival cases that completed three times monitoring, the variation rate of biochemical indexes in the two groups were compared and analyzed: alkaline phosphatase (ALP). The effects of creatine kinase, serum calcium, aspartate aminotransferase, total bilirubin, albumin, lipase and serum phosphatase on biochemical indexes were as follows: the concentration of alkaline phosphatase increased, the concentration of creatine kinase increased, and the concentration of serum calcium decreased. Aspartate aminotransferase concentration increased, total bilirubin concentration decreased, albumin concentration decreased, lipase concentration increased, serum phosphorus concentration increased. The results of the comparison between the cured group and the dead group showed that the decrease of pancreatic function, the decrease of serum calcium concentration and the deficiency of albumin may be the main causes of the death of the dogs with CPV. During the continuous detection of CPV, the changes of the indexes may indicate the development direction of the disease. The results showed that alkaline phosphatase, creatine kinase, serum calcium, aspartate aminotransferase should be monitored in time during treatment. The changes of total bilirubin, albumin, lipase and serum phosphorus, especially the changes of pancreatic function, serum calcium and protein, may increase the treatment rate of CPV and reduce the mortality.
【学位授予单位】：河南科技大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：S858.292

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