钼硒单施与配施对铅胁迫下小白菜生长及生理特性的影响

发布时间：2024-04-03 02:42

　　重金属作为土壤污染的主要污染因子,严重影响到生态安全和人体健康。利用植物营养元素与重金属离子间的拮抗作用修复重金属污染土壤,成为重金属污染治理的一项重要技术手段。硒和铝是已被证实的重金属拮抗离子。本文通过盆栽试验研究了钼硒配施对不同铅污染水平(0、5、50mg·kg)上小白菜铅毒害的缓解作用。通过分析小白菜鲜重和干重、不同部位微量元素含量、抗氧化指标以及土壤相关元素含量的变化,初步探讨了钼硒缓解铅毒害的生理机制。主要研究结论如下： 1.低浓度的铅(5mg-kg)对小白菜的生长代谢视乎没有太大的影响,但是施用高浓度铅(50mg-kg)显著地抑制了小白菜的生长,降低了小白菜地上地下部的生物量。高浓度铅胁迫下,硒钼单施及配施均显著缓解了铅的毒害作用。施硒对正常生长条件下的小白菜产量有着显著地降低作用,但是施硒能显著地增加5mg kg-1及50mgkg-1两个铅水平上小白菜的产量。施铝增加了正常生长条件下小白菜的产量,但是却降低了50mg kg铅水平上小白菜的产量。钼硒配施的处理显著地增加了所有铅水平上小白菜的产量,甚至在50mg kg铅水平上小白菜的产量也达到了显著性水平。 2.随着土壤铅水...

【文章页数】：72 页

【学位级别】：硕士

【文章目录】：
摘要
Abstract
Abbreviations
CHAPTER 1
    1. Introduction
        1.1 Selenium
        1.2 Molybdenum
        1.3 Brassica campestris L. ssp. Pekinensis
        1.4 Literature review
            1.4.1 Pb as a threat to the environment
            1.4.2 Selenium and Molybdenum as potential antagonists
            1.4.3 Hyperaccumulator
    2. Background and objectives of the research
        2.1 Research main objectives
        2.2 Specific Objectives
        2.3 Research structure pathway
CHAPTER2 The effects of Se and Mo on the growth of Brassica campestris L. ssp. pekinensis underPb stress
    2.1 Introduction
    2.2 Materials and methods
        2.2.1 Soil used in this study
        2.2.2 Plant Material and growth condition
        2.2.3 Experiment Treatments
        2.2.4 Plant biomass
        2.2.5 Statistical Analysis
    2.3 Results
        2.3.1 Morphology
        2.3.2 Biomass
        3.3.3 Discussions
CHAPTER3 Effect of Se and Mo on the content of Pb in Brassica campestris L. ssp. Pekinensis tissuesunder Pb stress
    3.1 Introduction
    3.2 Materials and Methods
        3.2.1 Determination of Pb Content in shoots and roots
        3.2.2 Pb assay in the soil
        3.2.3 Statistical Analysis
    3.3 Results
        3.3.1 Pb content in the shoots and roots of Brassica campestris L. ssp. pekinensis
        3.3.2 Pb concentration in the soil
    3.4 Discussion
        3.4.1 Pb content in the shoots and roots of Brassica campestris L. ssp. Pekinensis
        3.4.2 Pb concentration in the soil
CHAPTER 4 Antagonistic interaction between Se and Mo under the influence of Pb stress in Brassicacampestris L. ssp. Pekinensis
    4.1 Introduction
    4.2 Materials and Methods
        4.2.1 Soil
        4.2.2 Assay of Se content in the shoots and roots
        4.2.3 Analysis of Mo content in the leafs and roots of
        4.2.4 Assay of Se concentration in the soil after experiment
        4.2.5 Assay of Mo concentration in the soil after experiment
    4.3 Statistical Analysis
    4.4 Results
        4.4.1 Se content in the shoots and roots of Brassica campestris L. ssp. Pekinensis
        4.4.2 Mo contents in the shoots and roots of Brassica campestris L. ssp. Pekinensis
        4.4.3 Se concentration in the soil after the experiment
        4.4.4 Mo concentration in the soil after the experiment
    4.5 Discussion
        4.5.1 Se content in the shoots and roots of Brassica campestris L. ssp. Pekinensis
        4.5.2 Mo contents in the shoots and roots of Brassica campestris L. ssp. Pekinensis
        4.5.3 Se concentration in the soil after the experiment
        4.5.4 Mo concentration in the soil after the experiment
CHAPTER 5 Effect of Se and Mo on the minerals elements uptake by Brassica campestris L. ssp.Pekinensis under Pb stress
    5.1 Introduction
    5.2 Materials and methods
        5.2.1 Determination micro-elements in shoots and roots
        5.2.2 Statistical Analysis
    5.3 Results
        5.3.1 Macro and micro-elements content in shoots and roots of Brassica campestris L. ssp. Pekinensis under Pb stress
    5.4 Discussion
CHAPTER6 Reaction of antioxidant enzymes activities on Se and Mo in attempt to alleviate Pb toxicity
    6.1 Introduction
    6.2 Materials and Methods
        6.2.1 Soil utilized in this study
        6.2.2 Plant materials
        6.2.3 Analysis of antioxidant enzymes activities:SOD, CAT and POD
        6.2.4 Analysis of antioxidant non-enzymes activities:AsA and GSH
        6.2.5 Statistical Analysis
    6.3 Results
        6.3.1 Analysis of antioxidant enzymes activities (SOD EC 1.15.1.1) in shoots of Brassica campestris L. ssp. pekinensis
        6.3.2 CAT EC 1.11.1.6 enzymes activity in the shoots of Brassica campestris L. ssp.pekinensis
        6.3.3 POD EC 1.11.1.7 Enzymes activity in the shoots of Brassica campestris L. ssp.pekinensis
    6.4 Non-enzymes antioxidants contents in the shoots of Brassica campestris L. ssp.pekinensis
        6.4.1 AsA contents
        6.4.2 GSH contents
    6.5 Discussion
CHAPTER 7
    7.1 conclusion
References
Acknowledgements



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