氧化应激在血糖波动加速GK大鼠糖尿病肾病发病过程中的作用

发布时间：2018-12-16 09:03

【摘要】：目的评价氧化应激(OS)在血糖波动加速DN发病过程中的作用。方法 16只雄性GK大鼠随机分为持续高血糖(MS)组与血糖波动(MF)组,另选择8只雄性Wistar大鼠作为正常对照(NC)组。MF组每日错时腹腔注射葡萄糖及皮下注射胰岛素造成血糖波动模型,MS组每日错时腹腔注射葡萄糖造成持续高血糖模型,NC组每日错时腹腔注射等剂量生理盐水,连续6周。每日观察大鼠的一般体征,第6周末检测全日9个时间点的血糖。麻醉处死大鼠,心脏采血,检测血肌酐(Scr)、血尿酸(SUA)、TC、TG等指标的含量;取双侧肾脏,称重,计算肾脏指数;检测肾组织与肾组织线粒体中OS水平,包括丙二醛(MDA)、超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)的含量;HE染色,光镜下观察大鼠肾脏病理变化。结果与NC组相比,MS组与MF组体重增长速度减慢,肾脏指数、Scr、SUA、TC、TG、肾组织与肾组织线粒体中MDA升高(P0.05或P0.01),肾组织与肾组织线粒体中SOD与GSH-Px降低(P0.05或P0.01),肾脏病理改变严重;与MS组相比,MF组肾脏指数、Scr、SUA、TC、肾组织与肾组织线粒体中MDA、SOD、GSH-Px等指标及肾脏病理上的变化更明显(P0.05或P0.01)。结论 OS水平在糖尿病大鼠肾组织与肾组织线粒体中增强,其在血糖波动加速DN发病过程中发挥重要作用。
[Abstract]:Objective to evaluate the role of oxidative stress (OS) in the pathogenesis of DN. Methods Sixteen male GK rats were randomly divided into hyperglycemic (MS) group and hyperglycemic fluctuating (MF) group. In addition, 8 male Wistar rats were selected as normal control (NC) group. Glucose fluctuation model was induced by intraperitoneal injection of glucose and subcutaneous injection of insulin in MF group, and sustained hyperglycemia model was induced by intraperitoneal injection of glucose in MS group. NC group received intraperitoneal injection of the same dose of saline at staggered times for 6 weeks. The general signs of the rats were observed daily and blood glucose was measured at 9 time points at the end of the 6th week. Anesthetized rats were killed, blood was collected from the heart, serum creatinine (Scr),) and serum uric acid (SUA), TC,TG) were measured, and bilateral kidneys were taken to weigh and calculate the renal index. The levels of OS, including malondialdehyde (MDA),) superoxide dismutase (SOD), glutathione peroxidase (GSH-Px) in mitochondria of kidney and kidney were detected, and the pathological changes of kidney were observed under light microscope with HE staining. Results compared with NC group, MS group and MF group had slower weight gain, lower renal index, and higher MDA in renal tissue and mitochondria of Scr,SUA,TC,TG, (P0.05 or P0.01). The levels of SOD and GSH-Px in mitochondria of kidney and kidney were decreased (P0.05 or P0.01), and the pathological changes of kidney were serious. Compared with MS group, the changes of renal index, MDA,SOD,GSH-Px in renal mitochondria and renal mitochondria in MF group were more obvious than those in MS group (P0.05 or P0.01). Conclusion the level of OS is increased in renal and renal mitochondria of diabetic rats, and it plays an important role in accelerating the pathogenesis of DN by blood glucose fluctuation.
【作者单位】：衢州市人民医院科教科;浙江中医药大学药学院;浙江中医院中药房;浙江中医药大学科研处;
【基金】：国家自然科学基金(81403373) 浙江省自然科学基金(Y2110738) 浙江省医药卫生科技计划项目(2013KYA221) 浙江省教育厅研究项目(Y201226284)
【分类号】：R587.2

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