蒙古扁桃药材不同提取物对高脂血症大鼠肝脏保护作用及机理的研究

发布时间：2018-01-13 00:00

本文关键词：蒙古扁桃药材不同提取物对高脂血症大鼠肝脏保护作用及机理的研究　出处：《中华中医药学刊》2017年02期 　论文类型：期刊论文

【摘要】：目的:探究蒙古扁桃药材不同提取物对高脂血症大鼠肝脏脂质过氧化、脂代谢酶的影响。方法:以乙酸乙酯、正丁醇和水提取蒙古扁桃种仁,得各部提取物。大鼠按体质量均衡随机分组,分别为空白组(10只)、模型组(10只)和给药组(乙酸乙酯组、正丁醇组、水提物组和阳性药组,每组10只),空白组给予普通饲料,模型组和给药组采用高脂饲料喂养法建立高脂模型。建模成功后,持续按前述方法饲养,空白组与模型对照组给予生理盐水,给药组分别以蒙古扁桃乙酸乙酯提取物、正丁醇提取物和水提取物灌胃给药,阳性药组灌胃血脂康。给药七周后计算大鼠肝脏系数,测定大鼠血清中的ALT、AST和肝脏组织中丙二醛(MDA)、超氧化物歧化酶(SOD)、谷胱甘肽(GSH)、谷胱甘肽过氧化物酶(GSH-Px)、腺苷脱氨酶(ADA)、肝酯酶(HL)、脂蛋白酯酶(LPL)、总脂酶(LA)。同时光镜观察肝脏的病理学变化。结果:与高脂模型组相比,正丁醇组能显著降低ADA、MDA,显著升高GSH、GSH-Px、LPL、LA;水提物组显著升高HL和LA。结论:蒙古扁桃药材正丁醇提取物对高脂血症大鼠的肝脏有一定的保护作用,其机理可能与正丁醇提取物能提高高脂血症大鼠肝脏的抗氧化能力和肝脏脂代谢酶的活性等有关。
[Abstract]:Objective: to investigate the effects of different extracts of Mongolian almond on lipid peroxidation and lipid metabolism enzymes in the liver of hyperlipidemic rats. Methods: Mongolian almond seeds were extracted with ethyl acetate, n-butanol and water. Rats were randomly divided into blank group (n = 10), model group (n = 10) and administration group (ethyl acetate group, n-butanol group, water extract group and positive drug group). Ten rats in each group were given normal diet, while the model group and drug administration group were fed with high-fat diet to establish high-fat model. After the model was successfully established, the model was continuously fed according to the above-mentioned method. The blank group and the model control group were given normal saline, and the control group were treated with ethyl acetate extract, n-butanol extract and water extract, respectively. After seven weeks of administration, the liver coefficients of rats were calculated, and the alt AST in serum and malondialdehyde (MDAA) and superoxide dismutase (SOD) in liver tissue were measured. Glutathione glutathione (GSH), glutathione peroxidase (GSH-PxN), adenosine deaminase (ADAA), liver esterase (HLN), lipoprotein esterase (LPLL). Results: compared with the hyperlipidemic model group, n-butanol group could significantly reduce ADA-MDAs and increase GSH-Px significantly. LPLLA; Water extract group significantly increased HL and LA.Conclusion: the n-butanol extract of Mongolian almond has a certain protective effect on the liver of hyperlipidemic rats. The mechanism may be related to the effect of n-butanol extract on the antioxidation ability of liver and the activity of lipid metabolizing enzyme in liver of hyperlipidemic rats.
【作者单位】：包头医学院药学院;湖南医药学院;
【分类号】：R285.5
【正文快照】： Protective Effects of Different Extracts of Amygdalus Mongolica onLiver of Hyperlipidemia RatsWU Peisai1,2,SHI Songli1,CHENG Xianghui1,ZHENG Qiannan1,WU Tong1,HE Xin1,CHANG Hong1(1.Baotou Medical College,Baotou 014040,Inner Mongolia,China;2.Hunan Univers

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