土家族麝针疗法“活血生新”治疗缺血性脑中风的作用机制研究

发布时间：2018-12-20 10:14

【摘要】：目的:探讨土家族麝针疗法治疗缺血性脑中风的作用机制,揭示其活血化瘀诱导血管新生的药理作用。方法:采用改良线栓法建立缺血性脑中风大鼠模型,以Zea-longa为1~3分的大鼠为研究对象,以土家族麝针疗法干预3个疗程后,TTC染色法观察其对大脑梗死灶体积的影响,激光散斑成像技术观察其对脑皮质区整体血流速度的影响,western-blot法测定血管内皮生长因子(VEGF)及其受体1(VEGFR1)的表达水平。结果:与正常组比较,缺血性脑中风大鼠脑梗死体积显著增加,脑皮质区血流速度明显减缓,脑组织中VEGF蛋白和VEGFR1蛋白表达水平均显著增加;经给予土家族麝针疗法治疗后,脑梗死体积显著降低,脑皮质区的血流速度显著加快,脑组织中VEGF蛋白和VEGFR1蛋白表达水平显著增加,且其影响随着疗程的延长更为显著。结论:土家族麝针疗法能显著改善缺血性脑中风所致的脑梗死体积,其"活血生新"的作用机制可能与改善脑皮质区的血流速度、增加血管内皮细胞生长因子及其受体表达水平有关。
[Abstract]:Objective: to explore the mechanism of treating ischemic cerebral apoplexy with Tujia musk deer acupuncture, and to reveal its pharmacological effect on angiogenesis induced by activating blood circulation and removing blood stasis. Methods: the rat model of ischemic stroke was established by modified suture embolism. The rats with Zea-longa as 1 ~ 3 were used as the research objects. After three courses of intervention with the acupuncture therapy of musk deer, the effect of TTC staining on the volume of cerebral infarction was observed. The effect of laser speckle imaging on global blood flow velocity in cerebral cortex was observed and the expression of vascular endothelial growth factor (VEGF) and its receptor 1 (VEGFR1) were measured by western-blot. Results: compared with the normal group, the volume of cerebral infarction was significantly increased, the blood flow velocity of cerebral cortex was slowed down, and the expression of VEGF protein and VEGFR1 protein were significantly increased in cerebral tissue of ischemic stroke rats. After the treatment, the volume of cerebral infarction was significantly decreased, the blood flow rate of cerebral cortex was significantly accelerated, and the expression of VEGF protein and VEGFR1 protein in brain tissue were significantly increased, and the effect was more significant with the prolongation of treatment. Conclusion: Tujia musk deer acupuncture therapy can significantly improve the volume of cerebral infarction caused by ischemic stroke, and its mechanism of "promoting blood circulation and generating new blood" may be related to the improvement of blood flow velocity in cerebral cortex. Increased expression of vascular endothelial growth factor and its receptor is related to.
【作者单位】：湖北民族学院医学院;湖北民族学院土家医药研究所;广州中医药大学;
【基金】：湖北省教育厅科学技术研究项目(中青年人才项目)(Q20161904)-土家族麝针疗法治疗缺血性脑中风大鼠的作用机制研究
【分类号】：R245

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