瘤胃丁酸灌注促进山羊瘤胃上皮乳头生长及其机理研究

发布时间：2025-03-30 03:28

　　先前研究表明,瘤胃丁酸灌注可上调上皮细胞细胞周期蛋白cyclin D1,但其分子机制尚未阐明。本文的目的是研究丁酸对瘤胃上皮乳头生长的影响及其分子机制,首次探讨了瘤胃丁酸灌注对山羊瘤胃上皮细胞增殖和细胞凋亡相关基因表达的影响。研究分为两部分:1,瘤胃丁酸灌注实验;2,瘤胃上皮细胞原代培养实验。1,瘤胃丁酸灌注实验二十四只装有瘤胃瘘管的健康山羊(4月龄;体重19.05±0.43kg)随机分成两组:B组(n=12,丁酸50ml 0.3 g/kg·BW/天经瘘管灌注到瘤胃)和C组(n=12,经瘘管注入相同剂量的缓冲液,但不含丁酸)。每天上午饲喂前1h灌注,实验期为28天。每组的四只山羊分别在最后一天灌注后的5、7和9小时被屠宰。采集瘤胃上皮样品,用游标卡尺检测瘤胃上皮形态上变化;Real-time PCR法检测基因表达。采集瘤胃液,用气象色谱法测定瘤胃内挥发性脂肪酸(SCFA)含量。结果表明,与对照组相比,丁酸灌注组1小时后瘤胃内丁酸浓度增加了 99.77%,且差异显著,并且持续了2.5h。瘤胃上皮形态学数据表明,与对照组相比,丁酸灌注组山羊前腹盲囊,腹囊和后腹盲囊在5h时的瘤胃上皮的长度分别...

【文章页数】：159 页

【学位级别】：博士

【文章目录】：
ABSTRACT
摘要
LIST OF ABBREVIATIONS
INTRODUCTION
CHAPTER 1 REVIEW OF LITERATURE
    PART-Ⅰ MAMMALIAN GUT
        1. GUT PHYSIOIOGY
        2. GUT BARRIER ITS COMPONENTS AND FUNCTION
        3. EPITHELIAL BARRIER
        4. MUCUS LAYER
        5. CHEMOKINE AND CYTOKINES
        6. PATTERN RECOGNITION RECEPTORS(PRRS)
        7. MICROFOID(M)CELL
        8. PANETH CELLS
        9. GUT IMMUNE SYSTEM (INTESTINAL MACROPHAGE, INTESTINAL T LYMPHOCYTES,INTESTINAL DENDRITIC CELLS) AND ANTIMICROBIAL PEPTIDES(AMP)
            9.1 Intestinal macrophages
            9.2 Intestinal T lymphocytes
            9.3 Intestinal Dendritic Cell
    PART-Ⅱ ANATOMY AND DEVELOPMENT OF THE RUMEN
        1. RUMEN ANATOMY AND STRUCTURE
        2. Rumen development
    PART-Ⅲ AN OVERVIEW OF RUMEN FUNCTION
        1. RUMEN FERMENTATION (SCFA PRODUCTION)
        2. TRANSPORTATION THROUGH RUMEN WALL
        3. ROLE OF MONOCARBOXYLATE TRANSPORTERS(MCT)
        4. ACTIVE TRANSPORT
        5. INTRAEPITHELIAL SCFA METABOLISM
        6. HEPATIC AND PERIPHERAL SCFA METABOLISM
        7. FATE OF BUTYRATE IN RUMEN
    PART-Ⅳ EFFECTS OF BUTYRATE ON GASTROINTESTINAL FUNCTION ANDEPTTHELIAL GROWTH
        1. EFFECTS OF BUTYRATE ON SCFAS ABSORPTION AND PH REGULATION
        2. EFFECTS OF BUTYRATE ON ANIMAL PERFORMANCE AND RUMEN GROWTH
        3. MECHANISM OF BUTYRATE ACTION ON EPITHELIAL PROLIFERATION:
            3.1 Direct pathway (Histone deacetylase inhibitor)
            3.2 Indirect pathway (Role of growth factors:insulin and IGF-1)
        4. EFFECTS OF BUTYRATE ON RUMEN PAPILLAE GROWTH
        5. ANTI-INFLAMMATORY EFFECT OF BUTYRATE
        6. APOPTOTIC EFFECT OF BUTYRATE ON CANCER CELLS
    PART-Ⅴ EFFECTS OF BUTYRATE ON EPITHELIAL GROWTH
        1. RUMEN EPITHELIAL PROLIFERATION
        2. POSSIBLE ROLE OF BUTYRATE IN CELL PROLIFERATION AND ASSOCIATED GENEEXPRESSION IN THE RUMEN EPITHELIUM
    PART-Ⅵ RUMINANT HUSBANDRY IN PAKISTAN
        1. HISTORY AND CURRENT SITUATIONS OF RUMINANT HUSBANDRY
            1.1 Nomadic
            1.2 Transhumant
            1.3 Household and sedentary
        2. OVINE AND BOVINE BREEDS AND THE AREA DISTRIBUTION
        3. GOAT STUDY IN PAKISTAN
    REFRENCES
CHAPTER 2 RUMINAL BUTYRATE INFUSION INDUCED PAPILLAE GROWTH IS BASEDON THE REGULATION OF PROLIFERATIVE AND APOPTOTIC GENES INRUMEN EPITHELIUM
    1. MATERIALS AND METHODS
        1.1 Ethical Approval
        1.2 Experimental design, Goat management and infusion method
        1.3 Sample Collection
        1.4 Determina tion of ruminal SCFA concentration
        1.5 Morphometric analysis of rumen papillae
        1.6 Histometric analysis of rumen papillae
        1.7 Quantitative Real-Time PCR
        1.8 Statistical analysis
    2. RESULTS
        2.1 Ruminal SCFA concentration
        2.2 Morphometric analysis of rumen papillae
        2.3 Histometric analysis of Papillae
        2.4 Growth performance of goats
        2.5 Epithelial growth and related gene expression
        2.6 Effect of butyrate on gene expression related to cell cycle and apoptosis
    3. DISCUSSION
        3.1 Ruminal fermentation pattern
        3.2 Morphometric and Histometric analyses of the rumen papillae
        3.3 Growth performance of goats
        3.4 Rumen epithelial proliferation and related gene expression
        3.5 Apoptosis-related gene expression
    SUMMARY
    REFRENCES
CHAPTER 3 IN VITRO EFFECT OF BUTYRATE ON GENE EXPRESSION ASSOCIATED CELLPROLIFERATION,CELL CYCLE INHIBITION AND APOPTOSIS
    1. MATERIALS AND METHODS
        1.1 Cell Culture
        1.2 Quantitative Real-Time PCR
        1.3 Statistical analysis
    2. RESULTS
    3. DISCUSSION
    SUMMARY
    REFRENCES
CHAPTER 4 COMPARATIVE STUDY ON RUMEN PAPILLAE MORPHOLOGY OF YANGZIRIVER GOAT WITH THREE PAKISTANI GOAT BREEDS VIZ KAMORI,PATERI AND TAPRI UNDER NATURAL GRAZING CONDITION
    1. MATERIAL AND METHODS
        1.1 Sample collection
        1.2 Rumen Papillae Morphology
    2. RESULTS
        2.1 Morphometric analysis of rumen papillae
        2.2 Stomach and rumen weight of of goat breeds
    3. DISCUSSION
    SUMMARY
    REFERENCES
GENERAL CONCLUSION
INNOVATION
DEDICATION
ACKNOWLEDGEMENTS



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