Single Nucleotide Polymorphisms and the Association Analysis

发布时间：2025-07-09 05:25

　　体重是家禽产业最重要的性状之一。生长受多种因素调控,如基因、激素、营养和环境。GH基因具有过重生理学功能,包括促进肌肉生长、调控脂肪的生长、骨骼发育和代谢。胰岛素样生长因子IGF-Ⅰ和IGF-Ⅱ基因对于总体体重和肌肉生长至关重要,它们被认为是与胰岛素具有相似结构的促有丝分裂多肽。京海黄鸡是以当地地方黄鸡资源为育种素材,根据传统遗传育种理论,采用常规育种和分子标记辅助选择,经7个世代培育而成的小型优质肉鸡新品种,京海黄鸡具有耐粗饲和抗逆性强的特点。由于之前研究检测到了许多与京海黄鸡生长和繁殖性状相关的SNPs位点,因此其被认为是具有发展前景的肉鸡新品种。在本研究中,我们检测了 GH、IGF-Ⅰ和IGF-Ⅱ基因中可能影响京海黄鸡生长和繁殖性状的突变位点,并分析了这些位点之间的相互作用。所有的试验鸡均孵化于同一天,笼养,自由采食。记录生长和繁殖数据,并收集母鸡血样。由公鸡和母鸡的目标器官中提取总RNA以研究基因表达模式,所有样本均随机选自饲养于江苏省京海禽业集团有限公司的同一批次的京海黄鸡。使用PCR、SSCP和qPCR技术分析吗目的基因的多态性、表达谱和表达规律,并分析它们与生长和繁殖性状的...

【文章页数】：139 页

【学位级别】：博士

【文章目录】：
DEDICATION
ABSTRACT
摘要
LIST OF ABBREVIATIONS & SYMBOLS
CHAPTER1: LITERATURE REVIEW
    1.1 INTRODUCTION
    1.2 THE CHICKEN GROWTH HORMONE GENE (GH)
        1.2.1 The GH, Somatotropic and Thyrotropic Axes
    1.3 THE INSULIN-LIKE GROWTH FACTOR-Ⅰ GENE (IGF-Ⅰ)
        1.3.1 The Insulin-like growth Factor-Ⅰ gene (IGF-Ⅰ) axis
    1.4 THE INSULIN-LIKE GROWTH FACTOR-Ⅱ GENE (IGF-Ⅱ)
        1.4.1 The Insulin-like growth Factor-Ⅱ gene (IGF-Ⅱ) axis
    1.5 THE GROWTH HORMONE (GH) AND BONE
        1.5.1 Effects of GH and IGF-Ⅰ on Longitudinal Bone Growth
        1.5.2 The physiological role of GH and IGF-Ⅰ for bone growth
    REFERENCE
CHAPTER 2: ASSOCIATION ANALYSIS WITH GROWTH AND REPRODUCTIVE TRAITS AND THE EXPRESSION PROFILES OF THE GH GENE IN JINGHAI YELLOW CHICKENS
    2.1 INTRODUCTION
    2.2 MATERIAL AND METHODs
        2.2.1 Population and samples collection
        2.2.2 DNA extraction and genotyping
        2.2.3 Primer design and PCR amplification
        2.2.4 Single-strand conformation polymorphism (SSCP) and sequencing
        2.2.5 Expression profiles analysis
        2.2.6 Statistical analysis
    2.3 RESULTS
        2.3.1 Polymorphisms of GH and genetic variation analysis
        2.3.2 Allele and genotype frequency of SNP in GH gene
        2.3.3 Association analysis with growth traits in CH gene
        2.3.4 Association analysis with reproductive traits in CH gene
        2.3.5 Association analysis of interaction effect for growth traits in CH gene
        2.3.6 Association analysis of interaction effect for reproductive traits
        2.3.7 Expression profile of GH gene in male and female Jinghai yellow chickens
        2.3.8 Expression regularity of GH gene in male and female Jinghai yellow chickens
    2.4 DISCUSSION
    REFERENCE
CHAPTER 3: ASSOCIATION ANALYSIS WITH GROWTH AND REPRODUCTIVE TRAITS AND THE EXPRESSION PROFILES OF THE IGF-Ⅰ GENE IN JINGHAI YELLOW CHICKENS
    3.1 INTRODUCTION
    3.2 MATERIAL AND METHODS
        3.2.1 Population and samples collection
        3.2.2 DNA extraction and genotyping
        3.2.3 Primer design and PCR amplification
        3.2.4 Single-strand conformation polymorphism (SSCP) and sequencing
        3.2.5 Expression profiles analysis
        3.2.6 Statistical analysis
    3.3 RESULTs
        3.3.1 Polymorphisms of IGF-Ⅰ and genetic variation analysis
        3.3.2 Allele and genotype frequencies of SNP in IGF-Ⅰ gene
        3.3.3 Association analysis with growth traits
        3.3.4 Association analysis with reproductive traits
        3.3.5 Expression profile of IGF-Ⅰ gene in male and female Jinghai yellow chickens
        3.3.6 Expression regularity of IGF-Ⅰ gene in male and female Jinghai yellow chickens
    3.4 DISCUSSION
    REFERENCE
CHAPTER 4: ASSOCIATION ANALYSIS WITH GROWTH AND REPRODUCTIVE TRAITS AND THE EXPRESSION PROFILES OF THE IGF-Ⅱ GENE IN JINGHAI YELLOW CHICKENS
    4.1 INTRODUCTION
    4.2 MATERIAL AND METHODS
        4.2.1 Population and samples collection
        4.2.2 DNA extraction and genotyping
        4.2.3 Primer design and PCR amplification
        4.2.4 Single-strand conformation polymorphism (SSCP) and sequencing
        4.2.5 Expression profiles analysis
        4.2.6 Statistical analysis
    4.3 RESULTS
        4.3.1 Polymorphisms of IGF-Ⅱ and genetic variation analysis
        4.3.2 Allele and genotype frequency of SNP in IGF-Ⅱ gene
        4.3.3 Association analysis with growth traits
        4.3.4 Association analysis with reproductive traits
        4.3.5 Association analysis of interaction effect for growth traits
        4.3.6 Association analysis of interaction effect for Reproductive traits
        4.3.7 Expression profiles of IGF-Ⅱ gene in male and female Jinghai yellow chickens
        4.3.8 Expression regularity of IGF-Ⅱ gene in male and female Jinghai yellow chickens
    4.4 DISCUSSION
    REFERENCE
CHAPTER 5: ASSOCIATION ANALYSIS OF INTERACTION EFFECT FOR GROWTH AND REPRODUCTIVE TRAITS AMONG THE DETECTED MUTATIONS IN THE GH, IGF-Ⅰ AND IGF-Ⅱ GENES IN JINGHAI YELLOW CHICKENS
    5.1 INTRODUCTION
    5.2 MATERIAL AND METHODS
        5.2.1 Statistical analysis
    5.3 RESULTS
        5.3.1 Association analysis of interaction effect for growth traits between detected mutations in the GH and IGF-Ⅰ genes
        5.3.2 Association analysis of interaction effect for growth traits between detected mutations in the GH and IGF-Ⅱ genes
        5.3.3 Association analysis of interaction effect for growth traits between detected mutations in the IGF-Ⅰ and IGF-Ⅱ genes
        5.3.4 Association analysis of interaction effect for reproductive traits between detected mutations in the GH and IGF-Ⅰ genes
        5.3.5 Association analysis of interaction effect for reproductive traits between detected mutations in the GH and IGF-Ⅱ genes
        5.3.6 Association analysis of interaction effect for reproductive traits between detected mutations in the IGF-Ⅰ and IGF-Ⅱ genes
        5.3.7 The comparison of expression profile between the GH and IGF-Ⅰ genes in male and female Jinghai yellow chickens
        5.3.8 The comparison of expression profile between the GH and IGF-Ⅱ genes in maleand female Jinghai yellow chickens
        5.3.9 The comparison of expression profile between the IGF-Ⅰand IGF-Ⅱ genes in maleand female Jinghai yellow chickens
        5.3.10 The comparison of expression profile between the GH and IGF-Ⅰ genes in Breastand Leg muscle for both male and female Jinghai yellow chickens
        5.3.11 The comparison of expression profile between the GH and IGF-Ⅱ genes in Breastand Leg muscle for both male and female Jinghai yellow chickens
        5.3.12 The comparison of expression profile between the IGF-Ⅰ and IGF-Ⅱ genes inBreast and Leg muscle for both male and female Jinghai yellow chickens
    5.4 DISGUSSION
    REFERENCE
CONCLUSION
LIST OF PUBLICATIONS



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